Staphylococcal enterotoxin B exposed to pregnant rats inhibits the hedgehog signaling pathway in thymic T lymphocytes of the offspring.

The Hedgehog (Hh) signaling pathway is involved in T cell differentiation and development and plays a major regulatory part in different stages of T cell development. A previous study by us suggested that prenatal exposure to staphylococcal enterotoxin B (SEB) changed the percentages of T cell subpopulation in the offspring thymus. However, it is unclear whether prenatal SEB exposure impacts the Hh signaling pathway in thymic T cells.

Prenatal Exposure of Rats to Staphylococcal Enterotoxin B Alters the Expression of Th1 and Th2 Cytokines via Decreased Methylation in the Spleen of Adult but not Neonatal Offspring.

Background: The methylation of IFN-γ and IL-4 genes is regarded as an epigenetic regulation that maintains the Th1 or Th2 phenotype.

Objective: To explore the influence of prenatal administration of the staphylococcal enterotoxin B (SEB) in pregnant rats, on the IFN-γ or IL-4 expression in the offspring spleen.

Methods: The SEB or PBS was administered intravenously to pregnant rats on the embryo-day 16.

[Immune Modulatory Effect of Outer Membrane Vesicles Derived from on Mouse Bone Marrow-Derived Dendritic Cells].

Objective: To study the effect of outer membrane vesicles (OMVs) derived from (ST) on the ultrastructural features and immune function of dendritic cells (DC).

Methods: Mice bone marrow cells were collected aseptically, and myeloid DC were generated by the combined induction and amplification with recombinant mouse granulocyte-macrophage colony-stimulating factor (GM-CSF) and recombinant mouse interleukin-4 (rm IL-4). Cell morphology was observed under inverted phase contrast microscope and the phenotype was identified with flow cytometry.

Exposure of pregnant rats to staphylococcal enterotoxin B increases offspring splenic Treg number and function via decreasing FoxP3 methylation.

Staphylococcus aureus is an infectious pathogen that is relatively common, but that can cause severe disease in pregnant women and their fetus. We previously demonstrated that exposing pregnant rats to staphylococcal enterotoxin B (SEB) altered splenic CD4/CD8 T cell frequencies in their offspring. Whether prenatal SEB exposure impacts Tregs in these offspring, however, remains to be determined.

Phenotypic and genetic characteristics of isolates from site-specific dental plaque in China.

is an important cariogenic microbe. The potential characteristics of isolates from site-specific dental plaque are still not clear. This study aimed to investigate the phenotypic and genetic characteristics of isolates from site-specific dental plaque in China.

Exposure of pregnant rats to staphylococcal enterotoxin B attenuates the response of increased Tregs to re-exposure to SEB in the thymus of adult offspring.

Regulatory T cells (Tregs) play an essential role during homeostasis and tolerance of the immune system. Based on our previous study that exposure of pregnant rats to staphylococcal enterotoxin B (SEB) can alter the percentage of CD4/CD8 subsets in the thymus of the offspring, in this study, we focus on the influence of exposure of pregnant rats to SEB on number, function and response of Tregs in the thymus of the offspring. Pregnant rats at gestational day of 16 were intravenously injected with 15 μg SEB and the thymuses of the neonatal and adult offspring were harvested for this study.

Prenatal exposure of staphylococcal enterotoxin B attenuates the development and function of blood regulatory T cells to repeated staphylococcal enterotoxin B exposure in adult offspring rats.

Staphylococcal enterotoxin B (SEB) is an extensively studied super-antigen. A previous study by us suggested that SEB exposure during pregnancy could alter the percentage of CD4 and CD8 T cells in the peripheral blood of neonatal offspring rats. It is unknown whether SEB exposure during pregnancy can influence the development of regulatory T cells (Tregs) in the peripheral blood of neonatal offspring rats.

Insight into the Effect of Small RNA on Mutacin IV in .

() is a serious microbe causing dental caries. Mutacin IV is an effective bacteriocin produced by to antagonize numerous non-mutans streptococcal species. However, the posttranscriptional regulation of mutacin IV remains unclear.

[Influence of Ureaplasma Urealyticum Infection on Testicular Tissue Structure and Secretion Function in Rats].

Objective: To study the influences of ureaplasma urealitycum (UU) infection on testicular tissue structure and secretion function in rats.

Methods: Forty clean grade male SD rats were randomly divided into the experiment group A (at 7 d after surgery), experiment group B (at 14 d after surgery), control group C (at 7 d after surgery) and control group D (at 14 d after surgery). There were 10 rats in each group.

[Effect of cordycepin on apoptosis and autophagy of tongue cancer cells in vitro and the molecular mechanism].

Objective: To study the effect of cordycepin on cell cycle, apoptosis and autophagy of human tongue cancer TCA-8113 cells and explore the mechanism of cordycepin for inhibiting the occurrence of tongue cancer.

Methods: CCK-8 method was used to assess the inhibitory effect of cordycepin on TCA-8113 cell proliferation in vitro. The cell cycle and cell apoptosis of TCA-8113 cells treated with different concentrations of cordycepin were analyzed using flow cytometry.

Staphylococcal enterotoxin B administration in pregnant rats alters the splenic lymphocyte response in adult offspring rats.

Background: Our previous study suggested that SEB exposure in pregnant rats could lead to the change of T cells subpopulation in both peripheral blood and thymus of the offspring rats. However, rarely is known about the influence of SEB exposure in pregnant rats on T cell subpopulation in the spleens of offspring rats.

Results: SEB was intravenously administered to the pregnant rats at gestational day 16 in this study.

Staphylococcal enterotoxin B administration during pregnancy imprints the increased CD4:CD8 T-cell ratio in the peripheral blood from neonatal to adult offspring rats.

Our previous study demonstrated that Staphylococcal enterotoxin B (SEB) administration during pregnancy could alter the percentage of T cells subpopulation in the thymus of the neonatal rats; however, little is known about the effect of maternal SEB administration during pregnancy on T cells subpopulation in the peripheral blood of the offspring rats. In the present study, pregnant rats at gestational day 16 were intravenously injected with 15 µg SEB. The present study found that prenatal exposure to SEB significantly decreased the percentages of CD8 T cells in the peripheral blood of both neonatal rats on the fifth day after delivery and the adult offspring rats.

Decreased Vβ8.2 T-cells in neonatal rats exposed prenatally to Staphylococcal enterotoxin B are further deleted by restimulation in an in vitro cultured thymus.

Staphylococcal enterotoxin B (SEB) administration during adulthood can cause the anergy or deletion of variable portion of the ? chain (V?)?expressing T cells. However, the effect of maternal SEB administration during pregnancy on the thymocytes of neonatal rats remains to be elucidated. In the present study, pregnant rats at gestational day 16 were intravenously injected with 15 µg SEB.

Staphylococcal enterotoxin B and α‑toxin induce the apoptosis of ECV304 cells via similar mechanisms.

Staphylococcal enterotoxin B (SEB) and α‑toxin produced by Staphylococcus aureus (S. aureus) are important in the pathogenesis of diseases. In the present study, we investigated the effects of SEB and α‑toxin on ECV304 cells.

Role of α-toxin-induced apoptosis of umbilical vein endothelial cells in vertical infection of Staphylococcus aureus L-form.

Objective: To investigate α-toxin-induced apoptosis of umbilical vein endothelial cells and explore its role in vertical infection of Staphylococcus aureus L-form.

Methods: HUV-EC-C cells exposed to different concentrations (0, 10, 30, 90, and 270 ng/ml) of α-toxin for different time lengths (0, 2, 4, 6, and 8 h) were examined for apoptosis using flow cytometry with Annexin V-PI staining. The levels of tumor necrosis factor-α (TNF-α) and the activities of, caspase-3 and caspase-8 in the cell culture were detected by ELISA and colorimetric method, respectively.

Teratogenicity of Staphylococcus aureus L-forms using a mouse whole-embryo culture model.

Our previous studies have suggested that Staphylococcus aureus L-forms are able to pass through the placental barrier of mice from the maternal side to the fetal body and affect fetal growth and development, but little is known about the direct influence of S. aureus L-forms on embryos during the critical period of organogenesis. Mouse embryos at gestational day 8.

[Effect of maternal staphylococcal enterotoxin B administration during pregnancy on CD3⁺ TCR Vβ8⁺T cells of adult offspring rats].

Objective: To investigate the influence of maternal staphylococcal enterotoxin B (SEB) administration during pregnancy on CD3⁺ TCR Vβ8⁺T cells of adult offspring rats.

Methods: Pregnant maternal rats at gestational day (GD) 16 were injected intravenously with 15 µg SEB in 0.2 ml PBS (SEB group), and the control rats receive the same volume of PBS.

Prenatal dehydration alters renin-angiotensin system associated with angiotensin-increased blood pressure in young offspring.

The renin-angiotensin system (RAS) has an important role in cardiovascular homeostasis. This study determined the influence of water deprivation during pregnancy on the development of the RAS in rats, and examined blood pressure (BP) in the adolescent offspring. Pregnant rats were water deprived for 3 days at late gestation, and we examined fetal cardiac ultrastructure, as well as heart angiotensin (Ang) II receptor protein and mRNA, liver angiotensinogen and plasma Ang II concentrations.

Ovine fetal hormonal and hypothalamic neuroendocrine responses to maternal water deprivation at late gestation.

Angiotensin II (Ang II), aldosterone, and arginine-vasopressin (AVP) are three major neuropeptides or hormones that are important in the control of body fluid regulation. Dehydration during pregnancy induces alterations in maternal-fetal fluid homeostasis. It is still not clear about effects and mechanisms of maternal water deprivation on fetal neuroendocrine and hormonal responses.

Low doses of nicotine-induced fetal cardiovascular responses, hypoxia, and brain cellular activation in ovine fetuses.

Prenatal exposure to nicotine is associated with a variety of adverse outcomes. The present study investigated the effect of low doses of nicotine during pregnancy on fetal blood gases, cardiovascular system, and cellular activation in the brain. Intravenous administration of nicotine 10 or 25 microg/kg into ewe did not affect maternal blood gases, blood pressure, and heart rate.

The effect of prenatal nicotine on mRNA of central cholinergic markers and hematological parameters in rat fetuses.

A number of studies have demonstrated the influence of nicotine on fetal development. This study determined the expression of choline acetyltransferase (ChAT), vesicular acetylcholine transporter (VAChT), and high-affinity choline transporter (CHT1) in the forebrain and hindbrain following chronic prenatal nicotine exposure in the rat fetus (maternal rats were subcutaneously injected with nicotine at different gestation periods). We also measured the effect of chronic nicotine exposure on fetal blood pO(2), pCO(2), pH, Na(+) and K(+) concentrations, as well as lactic acid levels.