Publications by authors named "Juncai Wang"

Background: Considering that the respective effects of obesity and hyperlipidemia on knee osteoarthritis (OA) have not been fully investigated, the purpose of this study was to determine the relationship of obesity or hyperlipidemia with the synovitis and structural abnormalities of knee OA, and the effect of obesity and hyperlipidemia on functional outcomes of total knee arthroplasty.

Methods: There were 99 OA patients without obesity and hyperlipidemia in Group 1, 100 OA patients only with obesity in Group 2, 98 OA patients only with hyperlipidemia in Group 3, and 97 OA patients with both obesity and hyperlipidemia in Group 4. Semi-quantitative synovial inflammatory markers were measured including effusion-synovitis, size and intensity of infrapatellar fat pad abnormality, and synovial proliferation score.

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Article Synopsis
  • Cadmium (Cd) is harmful to plant growth and development, and this study focuses on understanding its effects on Solanum nigrum L. through various analyses over a week under several Cd concentrations.
  • Experiment results showed that higher Cd levels reduced biomass and mineral balance, increased Cd accumulation, and affected photosynthesis and related proteins in the plant.
  • Additionally, transcriptomic and metabolomic analyses identified key genes and metabolites involved in nutrient transport and detoxification, highlighting critical metabolic pathways that help the plant cope with Cd stress.
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Introduction: is currently the rarest and most endangered species of orchids in China and has significant ornamental value. The mature seeds of are difficult to germinate owing to the absence of an endosperm and are highly dependent on mycorrhizal fungi for germination and subsequent development. However, little is known about the regulation mechanisms of symbiosis and symbiotic germination of seeds.

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Similar to other orchid species, (Lindl.ex Hook.) Stein, relies on nutrients provided by mycorrhizal fungus for seed germination and seedling development in the wild owing to a lack of endosperm in its seeds.

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In the age of nanotechnological advancement, carbon nanotubes (CNTs) are drawing global attention. However, few studies have been published on the crop growth responses to CNTs in heavy metal(loid)s contaminated environments. A pot experiment was conducted to assess the effect of multi-walled carbon nanotubes (MWCNTs) on plant development, oxidative stress, and heavy metal(loid)s behavior in a corn-soil system.

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Changes in altitude can cause regional microclimate changes, leading to the spatial heterogeneity of environmental factors and soil bacteria. However, the internal driving process and mechanism remain unclear. Here, we selected Fanjingshan, a typical nature reserve in the subtropical region of south China with a clear altitudinal belt, to reveal the response mechanisms of microbial populations with altitude changes.

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Article Synopsis
  • The study investigates the impact of multi-walled carbon nanotubes (MWCNTs) on the growth of Solanum nigrum L. (S. nigrum) under stress from cadmium and arsenic, revealing significant growth enhancements with 500 mg/kg of MWCNTs.
  • MWCNTs increased shoot length, root length, and fresh biomass by 1.38-fold, 1.56-fold, and 1.37-fold, respectively, while also improving nutrient absorption and enzyme activities.
  • Transcriptomic and metabolomic analyses identified gene expression changes and key metabolic pathways associated with defense and stress responses, highlighting MWCNTs' potential in soil remediation.
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Microorganisms affect cadmium (Cd) extraction by hyperaccumulators to varying degrees, but the potential mechanism has not been completely studied. Here, two plant growth-promoting bacteria (PGPB, Bacillus paranthracis NT1 and Bacillus megaterium NCT-2) were assessed for their influence on Cd uptake by Solanum nigrum L. and their influence mechanisms.

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Nitrate is the main nitrogen source for plant growth, but it can also pollute the environment. A major cause of soil secondary salinization is the rising level of nitrates in the soil, which poses a threat to the sustainability and fertility of global greenhouse soils. Herein, Bacillus megaterium NCT-2 was used as a microbial agent to remove nitrate by bioaugmentation, and the remediation efficiency of secondary salinized soil in different degrees was evaluated.

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Under the action of heat, the jet fire of oil-filled equipment in substations produces intense heat radiation and flame impact, causing serious harm to the personnel and equipment. Therefore, this paper has carried out a series of oil-filled equipment transformer oil jet fire experiments with different nozzle diameters to study the characteristics of transformer oil jet flame combustion. The experiment measured the mass loss rate, axial centerline temperature of the fire plume, and radiant heat flux and processed the flame visualization image.

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Cadmium (Cd) triggers molecular alterations in plants, perturbs metabolites and damages plant growth. Therefore, understanding the molecular mechanism underlying the Cd tolerance in plants is necessary for assessing the persistent environmental impact of Cd. In this study, Solanum nigrum was selected as the test plant to investigate changes in biomass, Cd translocation, cell ultrastructure, metabolites and genes under hydroponic conditions.

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Carbon nanotubes can potentially stimulate phytoremediation of heavy metal contaminated soil by promoting plant biomass and root growth. Yet, the regulating mechanism of carbon nanotubes on the rhizosphere microenvironment and their potential ecological risks remain poorly characterized. The purpose of this study was to systematically evaluate the effects of multi-walled carbon nanotubes (MCNT) on the diversity and structure of rhizosphere soil bacterial and fungal communities, as well as soil enzyme activities and nutrients, in Solanum nigrum L.

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Ti-Si-Cu coatings (TS-C,= 5, 10 and 15 wt.%) with advanced bio-corrosion resistance, excellent antibacterial property and biocompatibility were laser cladded on Ti-6Al-4V (TAV) substrate which is widely used as endosseous implants. The bio-corrosion resistance of the TAV substrate was improved due to the presence of TiSiand TiCu phases in the coatings.

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Cadmium (Cd) is a prevalent, non-essential, carcinogenic, and hazardous heavy metal that reduces plant productivity and capacity of arable land area around the globe. In the present substrate-based pot study, seedlings of 180015 were grown equidistantly in the spiked-substrate medium for 60 days under increasing concentrations of Cd (0, 10, 20, 30, 40, 50 mg kg). Following harvest, the morpho-physio-biochemical, antioxidative, and Cd-induced tolerance responses were evaluated in under an increasing Cd stress regime.

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With the fast development of nanotechnology, nanomaterials are being increasingly applied for the remediation of contaminated soils. However, few researches have been reported on the complex interactions of carbon nanotubes with heavy metal (loid)s in phytoremediation. Here, we conduct a pot experiment to investigate the effects of multi-walled carbon nanotubes (MWCNTs) on the plant growth and behavior of heavy metal (loid)s in hyperaccumulator-soil system.

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The influence of Streptomyces griseorubens JSD-1 on microbial community succession during rice straw composting in an industrial-scale fermenter was assessed by high-throughput sequencing technology. Compared to uninoculated control, JSD-1 inoculation effectively raised composting temperature and improved other maturation indices. JSD-1 inoculation increased the relative abundance of Actinobacteria in thermophilic phase and Firmicutes in cooling and maturation phases.

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Solanum nigrum is a well-documented cadmium (Cd) hyperaccumulator; however, its Cd-induced tolerance capability and detoxification mechanism remain elusive. Hence, a short-term hydroponic experiment was performed in a multiplane glasshouse to determine the influence of Cd toxicity on subcellular distribution, chemical forms, and the physiological responses of cell wall towards Cd stress in a 4-week-old plant. The experiment was conducted following completely randomized design (CRD) with five treatments (n = 4 replicates).

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In developing countries, Cd contamination is ubiquitous which limits agriculture productivity. The current study was designed to investigate the efficacy of plant--ethylene diamine tetraacetic acid (EDTA) and plant-microbe-chelator (PMC) synergy for enhanced plant growth and Cd-uptake potential of in industrially contaminated and cadmium (Cd) spiked soil. A pot experiment was conducted by growing seedlings either inoculated with or un-inoculated along with the application of 5mM EDTA.

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Soil salinity is a global threat to the environmental sustainability, in particular to the developing countries due to their limited resources for soil reclamation. In a greenhouse pot experiment, Pennisetum giganteum, was investigated for its tolerance to salt stress and simultaneous phytoremediation capability. 4 weeks post-germination, NaCl (10, 50, 150, 250, 350, 450 and 550 mM) and tap water (control) was applied after every 2 consecutive days for two weeks in a completely randomized design and their effects were established in the growth and physico-chemical aspects of these plants.

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In the current experiment, influence of NPK composition on the Cd contaminated soil-plant (Solanum nigrum L.) system as well as the phytoremediation efficiency were comprehensively studied. The composition of NPK was optimized for a sustainable phytoremediation and simultaneous agronomic technique in Cd-contaminated soil by orthogonal (L) experiment, aimed to achieve plant productivity and maximum phytoremediation potential enhancement.

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Recently, long noncoding RNAs (lncRNAs) have been shown to have critical regulatory roles in human cancer biology. LncRNA CCAT2 is a novel identified lncRNA that was previously reported to be up-regulated in different cancers, however, its role in prostate cancer remains unclear. The aim of this study was to investigate the expression and role of lncRNA CCAT2 in prostate cancer.

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Background And Aims: Cholesterol over-intake is involved in the onset of nonalcoholic steatohepatitis (NASH), and hepatocellular bile acid (BA) accumulation correlates with liver injuries. However, how dietary cholesterol influences cholesterol and BA kinetics in NASH liver remains ambiguous and needs to be clarified.

Methods: Molecular markers involved in cholesterol and BA kinetics were investigated at protein and mRNA levels in an already-established stroke-prone spontaneously hypertensive 5/Dmcr rat model with fibrotic steatohepatitis, by feeding a high fat-cholesterol (HFC) diet.

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Aims: Eicosapentaenoic acid (EPA) can ameliorate certain liver lesions involved in non-alcoholic steatohepatitis (NASH). A previous study has found that stroke-prone spontaneously hypertensive 5/Dmcr (SHRSP5/Dmcr) rats fed a high fat-cholesterol (HFC) diet developed fibrotic steatohepatitis with histological similarities to NASH. This study evaluated the potential effects and mechanisms of action of EPA supplementation using this rodent model.

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