Immunoaffinity Depletion of High-Abundance Proteins from Serum/Plasma for Proteomic Analysis.

Mass spectrometry-based proteomics is widely applied to human blood serum or plasma in the search of biomarkers for various diseases. However, the enormous complexity and dynamic range of protein concentrations in these samples render a significant analytical challenge, particularly for detecting low-abundance candidate biomarkers. As a result, strategies for enriching low-abundance proteins and improving their identification in serum or plasma proteomics are commonly used.

Plasma/serum proteomics: depletion strategies for reducing high-abundance proteins for biomarker discovery.

Plasma and serum are widely used for proteomics-based biomarker discovery. However, analysis of these biofluids is highly challenging due to the complexity and wide dynamic range of their proteomes. Notably, highly abundant proteins tend to obscure the detection of potential biomarkers that are usually of lower concentrations.

Sudden Cardiac Death (SCD) - risk stratification and prediction with molecular biomarkers.

Sudden cardiac death (SCD) is a sudden, unexpected death that is caused by the loss of heart function. While SCD affects many patients suffering from coronary artery diseases (CAD) and heart failure (HF), a considerable number of SCD events occur in asymptomatic individuals. Certain risk factors for SCD have been identified and incorporated in different clinical scores, however, risk stratification using such algorithms is only useful for health management rather than for early detection and prediction of future SCD events in high-risk individuals.

Simultaneous determination of 25-hydroxyvitamin D and 25-hydroxyvitamin D in human serum by ultra performance liquid chromatography: An economical and validated method with bovine serum albumin.

A simple and economical method has been developed for simultaneous determination of human serum 25-hydroxyvitamin D (25OHD) and 25-hydroxyvitamin D (25OHD) using Ultra Performance Liquid Chromatography (UPLC). Non-human matrix of 4% BSA was used to construct the calibration curve and in quality control samples' preparation to avoid interference of the endogenous 25-hydroxyvitamin D (25OHD) present in the human serum. 25OHD, 25OHD and dodecanophenone (internal standard, IS) were separated on a CORTECS solid-core particle column and monitored by photodiode array detector at wavelength of 265 nm within five min run time.

Quantity and quality assessment of DNA extracted from saliva and blood.

Background: Saliva has been suggested as an attractive resource for evaluating physiological and pathological conditions in humans. This study aims to evaluate saliva sampling as an alternative to blood sampling for molecular testing.

Methods: We compared the yield, purity, and performance of DNA isolated from blood to that isolated from saliva using the non-invasive collection kit (Oragene DNA OG500 and OG575 kit).