Trend of Mortality Due to Congenital Anomalies in Children Younger Than 5 Years in Eastern China, 2012-2021: Surveillance Data Analysis.

Background: As one of the leading causes of child mortality, deaths due to congenital anomalies (CAs) have been a prominent obstacle to meet Sustainable Development Goal 3.2.

Objective: We conducted this study to understand the death burden and trend of under-5 CA mortality (CAMR) in Zhejiang, one of the provinces with the best medical services and public health foundations in Eastern China.

Untargeted metabolomics based on ultra-high performance liquid chromatography-mass spectrometry/MS reveals the lipid-lowering mechanism of taurine in hyperlipidemia mice.

Introduction: Taurine has a prominent lipid-lowering effect on hyperlipidemia. However, a comprehensive analysis of the effects of taurine on endogenous metabolites in hyperlipidemia has not been documented. This study aimed to explore the impact of taurine on multiple metabolites associated with hyperlipidemia.

A real-world study on the changing characteristics of measles antibodies in premature infants in China.

The waning of maternal antibodies may cause infants to lose protection against measles before receiving measles-containing vaccine (MCV). The aim of this study is to investigate the changing characteristics and influencing factors of measles antibodies in preterm infants (PT), and to provide scientific basis for optimizing MCV vaccination strategy of the target population. Blood samples were collected from PT and full-term infants (FT) at the chronological age (CA) of 3, 6, and 12 months.

Artificial Periosteum with Oriented Surface Nanotopography and High Tissue Adherent Property.

Massive periosteal defects often significantly impair bone regeneration and repair, which have become a major clinical challenge. Unfortunately, current engineered periosteal materials can hardly currently focus on achieving high tissue adhesion property, being suitable for cell growth, and inducing cell orientation concurrently to meet the properties of nature periosteum. Additionally, the preparation of oriented surface nanotopography often relies on professional equipment.

Circulating Neuropeptide Y May Be a Biomarker for Diagnosing Atrial Fibrillation.

Introduction: Sympathetic nervous system disorder promotes atrial fibrillation (AF), and neuropeptide Y (NPY) is an important neurotransmitter. This study aimed to explore the predictive value of plasma NPY in patients with AF.

Methods: Five hundred seventy-six patients were divided into AF (including paroxysmal and long-standing persistent AF; 360) and sinus rhythm (SR) groups (216).

Analysis of the difference between early-bolting and non-bolting roots of Angelica dahurica based on transcriptome sequencing.

Angelica dahurica (Fisch. ex Hoffm.) Benth.

Real-world vaccination status of children with hematologic tumors before and after chemotherapy.

Background: There is a high incidence and mortality rate in children with hematologic tumors (CHT), who are more prone to various infectious diseases. This study aims to clarify the real-world National Immunization Program (NIP) vaccination status of CHT before and after chemotherapy.

Methods: Medical records, NIP vaccination data, and the Adverse Event Following Immunization (AEFI) of CHT who were admitted to the Children's Hospital, Zhejiang University School of Medicine, from 1 January 2011 to 1 December 2021 were completely collected.

The Proportion of Circulating CD45RO+CD8+ T Cells is Associated with the Coronary Slow Flow.

Background: Circulating memory CD8+ T cells have been shown to be a crucial mediator of chronic inflammation. This study investigated whether the baseline proportion of circulating CD45RO+CD8+ T cells was associated with the coronary slow flow (CSF) phenomenon.

Methods: A total of 160 consecutive patients [mean (standard deviation (SD)) age, 67.

Effects of fruits and vegetables on gut microbiota in a mouse model of metabolic syndrome induced by high-fat diet.

The aim of this study was to evaluate the effect of fruit and vegetable intake on gut microbiota using a mouse model of metabolic syndrome (MS) induced by a high-fat diet. Forty-eight male mice were randomly divided into four groups, control group (C), high-fat diet-fed model group (H), high fat plus low intake of fruits and vegetables diet-fed group (H.LFV), high fat plus high intake of fruits and vegetables diet-fed group (H.

Mangiferin Alleviates Postpartum Depression-Like Behaviors by Inhibiting MAPK Signaling in Microglia.

Postpartum depression (PPD), a severe mental health disorder, is closely associated with decreased gonadal hormone levels during the postpartum period. Mangiferin (MGF) possesses a wide range of pharmacological activities, including anti-inflammation. Growing evidence has suggested that neuroinflammation is involved in the development of depression.

Profiling complex volatile components by HS-GC-MS and entropy minimization software: An example on Ligusticum chuanxiong Hort.

Volatile oil, as an important bioactive fraction of medicinal herbs, is comprised of a diversity of compounds. At present, gas chromatography-mass spectrometry (GC-MS) is one of the mainstream approaches to profiling these complex components. However, GC-MS faces the major bottleneck in data analysis, such as co-elution of more than one compound, and interference caused by high background noise; this usually makes an operator have to spend a lot of time and effort in optimizing experimental conditions.

[Effects of different field processing methods on volatile components of Chuanxiong Rhizoma: an exploration based on headspace gas chromatography-mass spectrometry].

The volatile oil of Chuanxiong Rhizoma(CX) is known as an effective fraction. In order to seek a suitable method for processing CX and its decoction pieces, this study selected 16 volatile components as indices to investigate how different processing methods such as washing/without washing, sun-drying, baking, oven-drying and far-infrared drying at different temperatures affected the quality of CX and its decoction pieces(fresh CX was partially dried, cut into pieces, and then dried) by headspace gas chromatography-mass spectrometry(GC-MS), cluster analysis, principal component analysis and comprehensive weighted scoring. The results showed that the rapid washing before processing did not deteriorate the volatile components of CX.

Vaccination deferral among children with seizures in Zhejiang: influence, recommendation, safety and implications.

Objectives: Delayed vaccination in children with seizures was common in China. This study aims to describe the vaccination status, reasons for vaccination deferral, vaccination recommendations and the safety for these patients in Zhejiang.

Methods: 1539 children included were divided into febrile seizure (FS) group, epilepsy (EP) group, unclassified seizure group and other symptomatic seizure group.

LncRNA PVT1 knockdown alleviated ox-LDL-induced vascular endothelial cell injury and atherosclerosis by miR-153-3p/GRB2 axis via ERK/p38 pathway.

Background And Aims: LncRNA plasmacytoma variant translocation 1 (PVT1) plays a regulatory role in some cardiovascular diseases, but its role in atherosclerosis (AS) remains barely explored. The study aimed to investigate the effects of PVT1 on high fat diet-induced AS and its potential mechanisms.

Methods And Results: ApoE -/- mice were fed with high fat diet for 8 weeks to establish an AS model.

ABHD15 promotes cell viability, glycolysis, and inhibits apoptosis in cardiomyocytes under hypoxia.

Background And Aims: Myocardial infarction (MI) has been an important heart disease affecting human health. The aim of this study was to investigate the regulatory effect of abhydrolase domain containing 15 (ABHD15) on hypoxic cardiomyocytes.

Methods And Results: Hypoxic cardiomyocytes are commonly used as an vitro model for the study of MI.

Prediction of adverse events in patients with initially medically treated type A intramural hematoma.

Background: Prior studies provided limited data regarding natural history of initially medically treated type A intramural hematoma (IMH).

Objectives: To develop predictive models for adverse aorta-related events in patients with type A IMH.

Methods: We performed a retrospective pooled analysis of individual patient data, including baseline clinical and CT characteristics.

Fe/Ni bimetal and nitrogen co-doped porous carbon fibers as electrocatalysts for oxygen reduction reaction.

In this work, oxygen reduction reaction (ORR) electrocatalysts Fe/Ni bimetal and nitrogen co-doped porous carbon fibers (Fe/Ni-NPCF), derived from electrospun nanofiber, have been prepared. In order to optimize the ORR performance of one dimension (1D) catalysts, SiO were used as template to create the hierarchical porous structure. The morphology of Fe/Ni-NPCF was investigated by scanning electron microscope (SEM) and transmission electron microscope (TEM).

ZIF-67/PAN-800 bifunctional electrocatalyst derived from electrospun fibers for efficient oxygen reduction and oxygen evolution reaction.

Metal-organic frameworks (MOFs) derived materials have been used as promising eletrocatalysts. However, the aggregation and poor conductivity are still obstacles for those eletrocatalysts. Herein, an effective method has been developed to overcome this problem by in-situ growth of ZIF-67 nanocrystals on the PAN fibers (ZIF-67/PAN) followed by the pyrolysis of ZIF-67/PAN fiber in 800 °C (ZIF-67/PAN-800).

Flexible, Porous, and Metal-Heteroatom-Doped Carbon Nanofibers as Efficient ORR Electrocatalysts for Zn-Air Battery.

Developing an efficient and durable oxygen reduction electrocatalyst is critical for clean-energy technology, such as fuel cells and metal-air batteries. In this study, we developed a facile strategy for the preparation of flexible, porous, and well-dispersed metal-heteroatom-doped carbon nanofibers by direct carbonization of electrospun Zn/Co-ZIFs/PAN nanofibers (Zn/Co-ZIFs/PAN). The obtained Zn/Co and N co-doped porous carbon nanofibers carbonized at 800 °C (Zn/Co-N@PCNFs-800) presented a good flexibility, a continuous porous structure, and a superior oxygen reduction reaction (ORR) catalytic activity to that of commercial 20 wt% Pt/C, in terms of its onset potential (0.

NDUFA4L2 protects against ischaemia/reperfusion-induced cardiomyocyte apoptosis and mitochondrial dysfunction by inhibiting complex I.

Myocardial ischaemia/reperfusion (I/R) injury may cause the apoptosis of cardiomyocytes as well as mitochondrial dysfunction. The aims of the present study were to investigate whether NADH dehydrogenase 1 alpha subcomplex subunit 4-like 2 (NDUFA4L2) on myocardial ischaemia-reperfusion (I/R) injury and the underlying molecular mechanism. The hypoxia-reperfusion (H/R) model was established in vitro using H9c2 cells to simulate I/R injury.

Cholesterol-lowing effect of taurine in HepG2 cell.

Background: A number of studies indicate that taurine promotes cholesterol conversion to bile acids by upregulating CYP7A1 gene expression. Few in vitro studies are concerned the concentration change of cholesterol and its product of bile acids, and the molecular mechanism of CYP7A1 induction by taurine.

Methods: The levels of intracellular total cholesterol (TC), free cholesterol (FC), cholesterol ester (EC), total bile acids (TBA) and medium TBA were determined after HepG2 cells were cultured for 24/48 h in DMEM supplemented with taurine at the final concentrations of 1/10/20 mM respectively.