Dynamic Monitoring of Organelle Interactions in Living Cells via Two-Color Digitally Enhanced Stimulated Emission Depletion Super-resolution Microscopy.

One of the most significant advances in stimulated emission depletion (STED) super-resolution microscopy is its capacity for dynamic super-resolution imaging of living cells, including the long-term tracking of interactions between various cells or organelles. Consequently, the multicolor STED plays a pivotal role in biological research. Despite the emergence of numerous fluorescent probes characterized by low toxicity, high stability, high brightness, and exceptional specificity, enabling dynamic imaging of living cells with multicolor STED, practical implementation of multicolor STED for live-cell imaging is influenced by several factors.

Magnetic field-induced synergistic therapy of cancer using magnetoplasmonic nanoplatform.

Combining photothermal and chemotherapy using single nanoplatform is an emerging direction in cancer nanomedicine. Herein, a magnetic field (MF) induced combination of chemo/photothermal therapy is demonstrated using FeO@mSiO@Au core@shell@satellites nanoparticles (NPs) loaded with chemotherapeutic drug doxorubicin (DOX), both and An application of an external MF to the NPs dispersion causes magnetophoretic movement and aggregation of the NPs. While the synthesized NPs only slightly absorb light at ∼800 nm, their aggregation results in a significant near infrared (NIR) absorption associated with plasmon resonance coupling between the Au satellites in the NPs aggregates.

Phasor-FSTM: a new paradigm for multicolor super-resolution imaging of living cells based on fluorescence modulation and lifetime multiplexing.

Multicolor microscopy and super-resolution optical microscopy are two widely used techniques that greatly enhance the ability to distinguish and resolve structures in cellular imaging. These methods have individually transformed cellular imaging by allowing detailed visualization of cellular and subcellular structures, as well as organelle interactions. However, integrating multicolor and super-resolution microscopy into a single method remains challenging due to issues like spectral overlap, crosstalk, photobleaching, phototoxicity, and technical complexity.

Multidimensional Characterization of the Physiological State of Hematococcuspluvialis Using Scanning Structured Illumination Super-Resolution Microscopy.

(HP) is a freshwater alga known for its ability to accumulate the potent antioxidant astaxanthin, which has extensive applications in aquaculture, pharmaceuticals, and cosmetics. Astaxanthin rapidly accumulates under unfavorable environmental conditions. However, the mechanisms of astaxanthin accumulation under various stress conditions remain unclear.

Near-infrared light therapy normalizes amyloid load, neuronal lipid membrane order, rafts and cholesterol level in Alzheimer's disease.

Cholesterol dysregulation, disorder of neuronal membrane lipid packing, and lipid rafts lead to the synthesis and accumulation of toxic amyloid-β (Aβ), contributing to the development of Alzheimer's disease (AD). Our study shows that near-infrared (NIR) transcranial photobiomodulation therapy (tPBMT) can reduce Aβ load and restore the properties of neuronal plasma membrane, including Aβ production, bilayer order, rafts, lipid content, and Ca channels during AD. Mice in the experiments were exposed to 808-nm LED for 1 h daily over 3 months.

In Vivo Neurodynamics Mapping via High-Speed Two-Photon Fluorescence Lifetime Volumetric Projection Microscopy.

Monitoring the morphological and biochemical information of neurons and glial cells at high temporal resolution in three-dimensional (3D) volumes of in vivo is pivotal for understanding their structure and function, and quantifying the brain microenvironment. Conventional two-photon fluorescence lifetime volumetric imaging speed faces the acquisition speed challenges of slow serial focal tomographic scanning, complex post-processing procedures for lifetime images, and inherent trade-offs among contrast, signal-to-noise ratio, and speed. This study presents a two-photon fluorescence lifetime volumetric projection microscopy using an axially elongated Bessel focus and instant frequency-domain fluorescence lifetime technique, and integrating with a convolutional network to enhance the imaging speed for in vivo neurodynamics mapping.

Tm-Based Downshifting Nanoprobes with Enhanced Luminescence at 1680 nm for In Vivo Vascular Growth Monitoring.

Optical imaging in the 1500-1700 nm region, known as near-infrared IIb (NIR-IIb), shows potential for noninvasive in vivo detection owing to its ultrahigh tissue penetration depth and spatiotemporal resolution. Rare earth-doped nanoparticles have emerged as widely used NIR-IIb probes because of their excellent optical properties. However, their downshifting emissions rarely exhibit sufficient brightness beyond 1600 nm.

Biopharmaceutical drug delivery and phototherapy using protein crystals.

Biopharmaceutical drugs, including proteins, peptides, and antibodies, are renowned for their high specificity and efficacy, fundamentally transforming disease treatment paradigms. However, their structural complexity presents challenges for their formulation and delivery. Protein crystals, characterized by high purity, high stability and a porous structure for biopharmaceutical drug encapsulation, providing a potential avenue for formulating and delivering biopharmaceutical drugs.

Correction: Bioactivity of cerium dioxide nanoparticles as a function of size and surface features.

Correction for 'Bioactivity of cerium dioxide nanoparticles as a function of size and surface features' by Veronika Sarnatskaya , , 2024, , 2689-2704, https://doi.org/10.1039/D3BM01900D.

SERS-Encoded Nanoprobes Based on Silver-Coated Gold Nanorods for Cell Sorting.

Optically-encoded probes have great potential for applications in the fields of biosensing and imaging. By employing specific encoding methods, these probes enable the detection of multiple target molecules and high-resolution imaging within the same sample. Among the various encoding methods, surface-enhanced Raman scattering (SERS) spectral encoding stands out due to its extremely narrow linewidth.

Erratum: Functionalized MoS Nanosheets as Multi-Gene Delivery Vehicles for Pancreatic Cancer Therapy: Erratum.

[This corrects the article DOI: 10.7150/ntno.27308.

Deep learning assisted femtosecond laser-ablation spark-induced breakdown spectroscopy employed for rapid and accurate identification of bismuth brass.

Background: Owing to its excellent machinability and less toxicity, bismuth brass has been widely used in manufacturing various industrial products. Thus, it is of significance to perform rapid and accurate identification of bismuth brass to reveal the alloying properties. However, the analytical lines of various elements in bismuth brass alloy products based on conventional laser-induced breakdown spectroscopy (LIBS) are usually weak.

Addressable scanning multifocal structured illumination microscopy using acousto-optic deflectors.

Multifocal structured illumination microscopy (MSIM) is a popular super-resolution imaging technique known for its good probe compatibility, low laser power requirements, and improved imaging depth, making it widely applicable in biomedical research. However, the speed of MSIM imaging is typically constrained by the approaches employed to generate and scan the laser foci across the sample. In this study, we propose a flexible two-photon excitation MSIM method using a pair of acousto-optic deflectors.

Overcoming photon and spatiotemporal sparsity in fluorescence lifetime imaging with SparseFLIM.

Fluorescence lifetime imaging microscopy (FLIM) provides quantitative readouts of biochemical microenvironments, holding great promise for biomedical imaging. However, conventional FLIM relies on slow photon counting routines to accumulate sufficient photon statistics, restricting acquisition speeds. Here we demonstrate SparseFLIM, an intelligent paradigm for achieving high-fidelity FLIM reconstruction from sparse photon measurements.

Optical Transparency Windows in Near-Infrared and Short-Wave Infrared for the Skin, Skull, and Brain: Fluorescence Bioimaging Using PbS Quantum Dots.

Fluorescence imaging (FI) employing near-infrared (NIR) light within the range of ~750-1350 nm enables biomedical imaging several millimeters beneath the tissue surface. More recent investigations into the short-wave IR (SWIR) transparency windows between ~1550-1870 and 2100-2300 nm highlight their superior capabilities. This research presents a comparison of IR-FI of PbS quantum dots, emitting at 990, 1310, and 1580 nm, through the mouse scalp skin, skull, and brain.

Single-shot spatial light interference microscopy for dynamic monitoring of membrane fluctuations.

Single-shot spatial light interference microscopy (SS-SLIM) with a pair of non-polarizing beam splitters is proposed for substantially enhancing the speed and efficiency of conventional SLIM systems. Traditional methods are limited by the need for multiple-frame serial modulation and acquisition by spatial light modulators and detectors. Our approach integrates non-polarizing beam splitters to simultaneously capture four phase-shifted intensity images, increasing the imaging speed by at least fourfold while maintaining high quality.

Ultrafast Laser 3D Nanolithography of Fiber-Integrated Silica Microdevices.

Fiber-integrated micro/nanostructures play a crucial role in modern industry, mainly owing to their compact size, high sensitivity, and resistance to electromagnetic interference. However, the three-dimensional manufacturing of fiber-tip functional structures beyond organic polymers remains challenging. It is essential to construct fiber-integrated inorganic silica with designed functional nanostructures for microsystem applications.

Research of Pulmonary Fibrosis Lesions Based on FLIM and SHG Imaging Microscopy.

Two-photon fluorescence lifetime microscopy (TP-FLIM) is a powerful quantitative imaging technique that characterizes and analyzes the structure and function of biological samples through a combination of intensity and lifetime imaging. Because TP-FLIM is independent of the fluorescence signal intensity and the fluorophore concentration, it is widely used in high-throughput, high-content drug screening and clinical diagnostics. Second harmonic generation (SHG) imaging technology has the advantages of high spatial resolution and imaging depth inherent to nonlinear optical imaging.

Bioactivity of cerium dioxide nanoparticles as a function of size and surface features.

Nano-dispersed cerium dioxide is promising for use in medicine due to its unique physicochemical properties, including low toxicity, the safety of usage, active participation in different redox processes occurring in living cells, and its regenerative potential, manifested in the ability of CeO to participate repeatedly in redox reactions. In this work, we examined the biological activity of cerium dioxide nanoparticles (CeO NPs) synthesized by precipitation in mixed water/alcohol solutions at a constant pH of 9. This synthesis method allowed controlling the size and Ce/Ce proportion on the surface of NPs, changing the synthesis conditions and obtaining highly stable suspensions of "naked" CeO NPs.