A high-contrast NIR excitation probe for monitoring Cu in the endoplasmic reticulum for synergistic cuproptosis and ferroptosis anticancer therapy.

Currently, the study of cuproptosis focuses on the Cu-induced morphology changes in mitochondria (Mito), and the observation of the effect of endoplasmic reticulum (ER)-related Cu content on cuproptosis is relatively lacking. Herein, we have developed a hydroxyflavone (HF)-based NIR excited two-photon fluorescent probe, BHCO, that exhibits specific recognition of Cu with high resolution. BHCO-Cu (Cu2BC) can lead to DLAT protein aggregation, triggering cuproptosis.

Functional abnormalities of the glymphatic system in cognitive disorders.

Various pathological mechanisms represent distinct therapeutic targets for cognitive disorders, but a balance between clearance and production is essential for maintaining the stability of the brain's internal environment. Thus, the glymphatic system may represent a common pathway by which to address cognitive disorders. Using the established model of the glymphatic system as our foundation, this review disentangles and analyzes the components of its clearance mechanism, including the initial inflow of cerebrospinal fluid, the mixing of cerebrospinal fluid with interstitial fluid, and the outflow of the mixed fluid and the clearance.

Ferulic acid mediates microbial fermentation of arabinoxylan to enhance host immunity by suppressing TLR4/NF-κB signaling.

The study was conducted to explore the relationship between arabinoxylan (AX) structure and microbial fermentation characteristics, and reveal molecular mechanism of AX on regulating immune function of the host. Results indicated that the group of wheat bran AX showed greater activity of feruloyl esterase, production of short chain fatty acids and ferulic acid compared with the blank group (P < 0.05).

The unexpected PD-L1 suppression function of celery-derived extracellular vesicles improves lung cancer chemotherapy efficacy.

The article explores celery-derived extracellular vesicles (CDEVs), characterized by high cellular uptake, low immunogenicity, and high stability, as a therapeutic strategy for antitumor nanomedicines. The methods employed in this study include cell experiments such as co-culture, Western Blot, and flow cytometry. experiments were conducted in C57BL/6 tumor-bearing mice subcutaneously injected with Lewis lung carcinoma (LLC) cells.

Intra-arterial tenecteplase after successful endovascular recanalisation in patients with acute posterior circulation arterial occlusion (ATTENTION-IA): multicentre randomised controlled trial.

Objective: To assess whether intra-arterial tenecteplase administered after successful endovascular recanalisation improves outcomes in patients with acute arterial occlusion of the posterior circulation.

Design: Multicentre randomised controlled trial.

Setting: 31 hospitals in China, 24 January 2023 to 24 August 2023.

The Effects of Moderate to High Static Magnetic Fields on Pancreatic Damage.

Background: Pancreatic damage is a common digestive system disease with no specific drugs. Static magnetic field (SMF), the key component of magnetic resonance imaging (MRI), has demonstrated prominent effects in various disease models.

Purpose: To study the effects of 0.

Maternal milk fat globule membrane enriched gut and circulating SCFAs to improve placental efficiency and fetal development in intrauterine growth restricted mice model.

Intrauterine growth restriction (IUGR) caused by placental dysfunctions leads to fetal growth defects. Maternal microbiome and its metabolites have been reported to promote placental development. Milk fat globule membrane (MFGM) is known for its diverse bioactive functions, while the effects of gestational MFGM supplementation on the maternal gut microbiota, placental efficiency, and fetal development remained unclear.

Glucose-Responsive Zn(II)-Porphyrin COF Adhesive Hydrogels With Dual-Active Sites and GOX-Like Activity for Accelerated Wound Healing.

Effective glycemic control is paramount for optimal wound healing in diabetic patients. Traditional antibacterial and anti-inflammatory treatments, while important, often fall short in addressing the hyperglycemic conditions of diabetic wounds. Therefore, the development of novel therapeutic strategies for accelerating diabetic wound healing has garnered escalating attention.

Identification and characterization of a novel chromosome-encoded aminoglycoside -nucleotidyltransferase gene, in sp. TYF-12 isolated from the marine fish intestine.

Background: The mechanisms underlying the resistance of the genus to aminoglycosides are complex, which poses a challenge for the efficient treatment of infectious diseases caused by these pathogens. To help clinicians treat infections more effectively, a more comprehensive understanding of antibiotic resistance mechanisms is urgently needed.

Methods: Plates were streaked to isolate bacteria from the intestinal contents of fish.

Atherosclerotic plaque evolution predicts cerebral ischemic events in patients with intracranial atherosclerosis: a multicentre longitudinal study using high-resolution MRI.

Background: Intracranial atherosclerosis (ICAS) is the leading cause of ischemic stroke in Asians and the recurrent rate remains high despite the optimal medical treatment. This study aimed to confirm that follow-up high-resolution magnetic resonance imaging (hrMRI) provided essential values in predicting subsequent cerebral ischemic events in patients with ICAS.

Methods: Patients with moderate to severe stenosis in the middle cerebral artery (MCA) defined by magnetic resonance (MRA) or computed tomography angiography (CTA) were recruited from three centers retrospectively.

Efficiency of combination therapy versus monotherapy for the treatment of infections due to carbapenem-resistant Gram-negative bacteria: a systematic review and meta-analysis.

Background: For resistant Gram-positive bacteria, evidence suggests that combination therapy is more effective. However, for resistant Gram-negative bacteria, no consensus has been reached. This study aims to comprehensively summarize the evidence and evaluate the impact of combination versus monotherapy on infections caused by carbapenem-resistant Gram-negative bacteria (CRGNB).

Molecular Engineering to Construct MoS with Expanded Interlayer Spacing and Enriched 1T Phase for "Rocking-Chair" Aqueous Calcium-Ion Pouch Cells.

The moderate working voltage and high capacity of transition metal dichalcogenides (TMDs) make them promising anode materials for aqueous calcium-ion batteries (ACIBs). However, the large radius and two charges of Ca cause TMDs to exhibit poor performance in ACIBs. Therefore, effective regulation strategies are crucial for enabling the application of TMDs in ACIBs.

Modulating Aggregation and Deaggregation Based on Assembling Strategy to Switch on NIR-II Light-Excited Fluorescence for Self-Reporting Viability of Eliminating Cancer Cell.

The fabrication of self-reporting photosensitizers (PSs), enabling real-time evaluation of the extent of elimination of cancer cells, holds significant scientific importance in the photodynamic therapy (PDT) process. To address the intrinsic challenge of the short-wavelength light source, this work proposed an innovative approach of rational design second near-infrared (NIR-II, 1000-1700 nm) light-excited fluorescent PS systems (named , , and , respectively) through modulating aggregation and deaggregation based on assembling strategy. Therein, the suitable interplanar distance of adjacent linked with C-H···π interactions was an idea for relieving compact π···π packing for fluorescent imaging as well as elevating the spin-orbit coupling for reactive oxygen species (ROS) generation.

Mechanistic insight of fungal-microalgal pellets in photobioreactor for heavy-metal wastewater bioremediation.

The high cost of harvesting microalgae limits their industrial application. Fungal-microalgal pellets can efficiently harvest microalgae and enhance heavy-metal adsorption. However, the molecular response mechanism of fungal-microalgal pellets under heavy-metal stress remains unclear.

Alzheimer's Disease-Derived Outer Membrane Vesicles Exacerbate Cognitive Dysfunction, Modulate the Gut Microbiome, and Increase Neuroinflammation and Amyloid-β Production.

Although our understanding of the molecular biology of Alzheimer's disease (AD) continues to improve, the etiology of the disease, particularly the involvement of gut microbiota disturbances, remains a challenge. Outer membrane vesicles (OMVs) play a key role in central nervous system diseases, but the impact of OMVs on AD progression remains unclear. In this study, we hypothesized that AD-derived OMVs (OMVs) were a risk factor in AD pathology.

Galactooligosaccharides and Limosilactobacillus reuteri synergistically alleviate gut inflammation and barrier dysfunction by enriching Bacteroides acidifaciens for pentadecanoic acid biosynthesis.

Ulcerative colitis (UC) is a debilitating inflammatory bowel disease characterized by intestinal inflammation, barrier dysfunction, and dysbiosis, with limited treatment options available. This study systematically investigates the therapeutic potential of a synbiotic composed of galactooligosaccharides (GOS) and Limosilactobacillus reuteri in a murine model of colitis, revealing that GOS and L. reuteri synergistically protect against intestinal inflammation and barrier dysfunction by promoting the synthesis of pentadecanoic acid, an odd-chain fatty acid, from Bacteroides acidifaciens.

[Prokaryotic expression and purification of inorganic nitrogen transporters in wheat].

The nitrate transporter (NRT) and ammonium transporter (AMT) are crucial transmembrane proteins involved in the absorption, transport, and distribution of inorganic nitrogen in wheat. Obtaining NRT/AMT and preparing corresponding antibodies are conducive to probing into their tissue localization and comprehending the nitrogen utilization process in wheat. In this study, four genes (.

Facile Preparation of Three-Dimensional Cubic MnSe/CNTs and Their Application in Aqueous Copper Ion Batteries.

Transition metal sulfide compounds with high theoretical specific capacity and excellent electronic conductivity that can be used as cathode materials for secondary batteries attract great research interest in the field of electrochemical energy storage. Among these materials, MnSe garners significant interest from researchers due to its unique three-dimensional cubic structure and inherent stability. However, according to the relevant literature, the performance and cycle life of MnSe are not yet satisfactory.

Self-Adaptive Electrochemistry of Phosphate Cathodes toward Improved Calcium Storage.

Polyanion phosphates exhibit great potential as calcium-ion battery (CIB) cathodes, boasting high working voltage and rapid ion diffusion. Nevertheless, they frequently suffer from capacity decay with irreversible phase transitions; the underlying mechanisms remain elusive. Herein, we report an adaptively layerized structure evolution from discrete NaVO(PO)F nanoparticles (NPs) to interconnected VOPO nanosheets (NSs), triggered by electrochemical (de)calcification, leading to an improvement in Ca storage performance.

C1-FDX is required for the assembly of mitochondrial complex I and subcomplexes of complex V in Arabidopsis.

C1-FDX (Complex I-ferredoxin) has been defined as a component of CI in a ferredoxin bridge in Arabidopsis mitochondria. However, its full function remains to be addressed. We created two c1-fdx mutants in Arabidopsis using the CRISPR-Cas9 methodology.