Predictive value of trendelenburg position and carotid ultrasound for fluid responsiveness in patients on VV-ECMO with acute respiratory distress syndrome in the prone position.

Fluid administration is widely used to treat hypotension in patients undergoing veno-venous extracorporeal membrane oxygenation (VV-ECMO). However, excessive fluid administration may lead to fluid overload can aggravate acute respiratory distress syndrome (ARDS) and increase patient mortality, predicting fluid responsiveness is of great significance for VV-ECMO patients. This prospective single-center study was conducted in a medical intensive care unit (ICU) and finally included 51 VV-ECMO patients with ARDS in the prone position (PP).

Research Progress in Shape-Control Methods for Wire-Arc-Directed Energy Deposition.

Wire-arc-directed energy deposition (WA-DED) stands out as a highly efficient and adaptable technology for near-net-shaped metal manufacturing, with promising application prospects. However, the shape control capability of this technology is relatively underdeveloped, necessitating further refinement. This review summarizes the latest advancements in the shape control of WA-DED technology, covering four pivotal areas: the regulation of various process parameters, optimization of the deposition paths, control through auxiliary energy and mechanical fields, and synergy between additive and subtractive manufacturing approaches.

Digitally Programmable Microphase Separation in Polymer Network Generates Microstructure Pattern.

Polymers with microstructure patterns are crucial to many applications, such as separation, optics, electronics, metamaterials, etc. However, introducing microstructure patterns with diverse morphologies and feature sizes ranging from nanometers to micrometers into large-area polymers remains a significant challenge. Here, we design a material system that enables digital programming microphase separation in a polymer network to generate microstructure patterns.

Irisin-regulated lncRNAs and their potential regulatory functions in chondrogenic differentiation of human mesenchymal stem cells.

Objective: Dysregulation of chondrogenic differentiation is associated with osteoarthritis (OA). The myokine irisin is beneficial in OA treatment; yet, the underlying mechanism is not fully understood. Long noncoding RNAs (lncRNAs) act as important regulators of chondrocyte differentiation.

Tertiary lymphoid structures potentially promote immune checkpoint inhibitor response in SMARCB1-deficient medullary renal cell carcinoma.

The WHO's classification of renal cell carcinoma (RCC) has identified loss of SMARCB1 as one of the driven mutations. Despite intensive postoperative interventions, the prognosis for SMARCB1-deficient medullary RCC remains poor, indicating insufficiency in current therapy. Herein, we reported the treatment outcomes of five patients with metastatic SMARCB1-deficient medullary RCC and molecular correlates.

[Research progress on bone repair biomaterials with the function of recruiting endogenous mesenchymal stem cells].

Objective: To review the research progress on bone repair biomaterials with the function of recruiting endogenous mesenchymal stem cells (MSCs).

Methods: An extensive review of the relevant literature on bone repair biomaterials, particularly those designed to recruit endogenous MSCs, was conducted, encompassing both domestic and international studies from recent years. The construction methods and optimization strategies for these biomaterials were summarized.

Structure-guided design and evaluation of CRM197-scaffolded vaccine targeting GnRH for animal immunocastration.

Immunocastration is a humane alternative to surgical castration for controlling population and estrous behaviors in animals. Gonadotropin-releasing hormone (GnRH), the pivotal initiating hormone of the hormonal cascade in mammals, is the optimal target for immunocastration vaccine development. Cognate antigen-mediated cross-linking of B cell receptors (BCRs) is a strong activation signal for B cells and is facilitated by repetitive surface organizations of antigens.

HCC control by lycorine-based restraining of the MiR-224-5p/COLEC10 axis.

Lycorine (LYC), as a natural alkaloid, possesses various significant biological activities. This study aims to investigate the impact and underlying mechanisms of LYC on the malignant progression of hepatocellular carcinoma (HCC). The levels of miR-224-5p, collectin subfamily member 10 (COLEC10) and inflammatory factors were quantified by RT-qPCR.

SurfFlow: A Flow-Based Approach for Rapid and Accurate Cortical Surface Reconstruction from Infant Brain MRI.

The infant brain undergoes rapid changes in volume, shape, and structural organization during the first postnatal year. Accurate cortical surface reconstruction (CSR) is essential for understanding rapid changes in cortical morphometry during early brain development. However, existing CSR methods, designed for adult brain MRI, fall short in reconstructing cortical surfaces from infant MRI, owing to the poor tissue contrasts, partial volume effects, and rapid changes in cortical folding patterns.

The clinical application of metagenomic next-generation sequencing in immunocompromised patients with severe respiratory infections in the ICU.

Background: Early targeted antibiotic therapy is crucial for improving the prognosis of immunocompromised patients with severe respiratory infections (SRIs) in the intensive care unit (ICU). Metagenomic next-generation sequencing (mNGS) has shown significant value in pathogen detection, but research on lower respiratory tract microorganisms remains limited.

Methods: This study enrolled 234 patients with SRIs in the ICU, and individuals were categorized into immunocompromised and immunocompetent groups.

Targeting PYK2 with heterobifunctional T6BP helps mitigate MASLD and MASH-HCC progression.

Background & Aims: The mechanisms underlying the regulation of hepatocyte non-receptor tyrosine kinases in metabolic dysfunction-associated steatohepatitis (MASH) remain largely unclear.

Methods: Hepatocyte-specific overexpression or deletion and anti-protein tyrosine kinase 2 beta (PYK2) or anti-TRAF6-binding protein (T6BP) crosslinking were utilized to study fatty liver protection by T6BP. A P-PTC (peptide-proteolysis targeting chimera) degrades PYK2 to block MASH progression.

African Swine Fever Virus Immunosuppression and Virulence-Related Gene.

African swine fever virus (ASFV), a highly contagious pathogen characterized by a complex structure and a variety of immunosuppression proteins, causes hemorrhagic, acute, and aggressive infectious disease that severely injures the pork products and industry. However, there is no effective vaccine or treatment. The main reasons are not only the complex mechanisms that lead to immunosuppression but also the unknown functions of various proteins.

Wrinkled metal-organic framework thin films with tunable Turing patterns for pliable integration.

Flexible integration spurs diverse applications in metal-organic frameworks (MOFs). However, current configurations suffer from the trade-off between MOF loadings and mechanical compliance. We report a wrinkled configuration of MOF thin films.

Epigenetic modification of PHLDA2 is associated with tumor microenvironment and unfavorable outcome of immune checkpoint inhibitor-based therapies in clear cell renal cell carcinoma.

Background: A substantial proportion of patients with metastatic clear cell renal cell carcinoma (ccRCC) cannot derive benefit from immune checkpoint inhibitor (ICI) plus anti-angiogenic agent combination therapy, making identification of predictive biomarkers an urgent need. The members of pleckstrin homology-like domain family A (PHLDA) play critical roles in multiple cancers, whereas their roles in ccRCC remain unknown.

Methods: Transcriptomic, clinical, genetic alteration and DNA methylation data were obtained for integrated analyses from TCGA database.

The Evolution of Grain Microstructure in Friction Stir Welding of Dissimilar Al/Mg Alloys with Ultrasonic Assistance.

The process of grain refinement during welding significantly influences both the final microstructure and performance of the weld joint. In the present work, merits of acoustic addition in the conventional Frictions Stir Welding (FSW) process were evaluated for joining dissimilar Al/Mg alloys. To capture the near "in situ" structure around the exit hole, an "emergency stop" followed by rapid cooling using liquid nitrogen was employed.

Standby extracorporeal membrane oxygenation: a better strategy for high-risk percutaneous coronary intervention.

Background: The incidence of cardiac arrest (CA) during percutaneous coronary intervention (PCI) is relatively rare. However, when it does occur, the mortality rate is extremely high. Extracorporeal cardiopulmonary resuscitation (ECPR) has shown promising survival rates for in-hospital cardiac arrests (IHCA), with low-flow time being an independent prognostic factor for CA.

A Real-Time Grasping Detection Network Architecture for Various Grasping Scenarios.

In the field of robot grasping detection, due to uncertain factors such as different shapes, distinct colors, diverse materials, and various poses, robot grasping has become very challenging. This article introduces a integrated robotic system designed to address the challenge of grasping numerous unknown objects within a scene from a set of α -channel images. We propose a lightweight and object-independent pixel-level generative adaptive residual depthwise separable convolutional neural network (GARDSCN) with an inference speed of around 28 ms, which can be applied to real-time grasping detection.

High-yield nanovesicles extruded from dental follicle stem cells promote the regeneration of periodontal tissues as an alternative of exosomes.

Aim: To identify an optimized strategy for the large-scale production of nanovesicles (NVs) that preserve the biological properties of exosomes (EXOs) for use in periodontal regeneration.

Materials And Methods: NVs from dental follicle stem cells (DFSCs) were prepared through extrusion, and EXOs from DFSCs were isolated. The yield of both extruded NVs (eNVs) and EXOs were quantified through protein concentration and particle number analyses.