Application of DNA Barcoding to Identify Medicinal Plants.

Medicinal plants are valuable resources globally and are used worldwide to maintain health and treat disease; however, the presence of adulteration obstructs their development. DNA barcoding, a technique for species identification by standard DNA regions, facilitates prompt and accurate identification of traditional medicinal plants. The process of DNA barcoding entails six basic steps: 1) processing the medicinal plants, 2) extracting high-quality total DNA from the medicinal plants using centrifugal column method, 3) amplifying target DNA region internal transcribed spacer 2 (ITS2) with universal primers of plants and performing Sanger sequencing, 4) splicing and aligning sequence to obtain the target sequence, 5) matching the barcode sequence against the barcode library for identification, 6) aligning sequence, comparing intraspecific and interspecific variation, constructing phylogenetic neighbor-joining tree.

Efficacy of intervertebral release combined with asymmetric osteotomy in rigid degenerative scoliosis.

Purpose: The aim of this study is to evaluate the clinical efficacy and safety of treating patients with rigid degenerative scoliosis by restoring intervertebral balance through a combination of interbody release and asymmetric grade 1 and 2 osteotomy.

Method: The medical collected clinical and radiographic data of patients with rigid degenerative scoliosis from our department between 2015 and 2022. A total of 60 patients were included in the study, comprising 20 males and 40 females, with an average follow-up period of 30.

Unveiling the Links Between Microbial Alteration and Host Gene Disarray in Crohn's Disease via TAHMC.

A compelling correlation method linking microbial communities and host gene expression in tissues is currently absent. A novel pipeline is proposed, dubbed Transcriptome Analysis of Host-Microbiome Crosstalk (TAHMC), designed to concurrently restore both host gene expression and microbial quantification from bulk RNA-seq data. Employing this approach, it discerned associations between the tissue microbiome and host immunity in the context of Crohn's disease (CD).

Development of UroSAM: A Machine Learning Model to Automatically Identify Kidney Stone Composition from Endoscopic Video.

Chemical composition analysis is important in prevention counseling for kidney stone disease. Advances in laser technology have made dusting techniques more prevalent, but this offers no consistent way to collect enough material to send for chemical analysis, leading many to forgo this test. We developed a novel machine learning (ML) model to effectively assess stone composition based on intraoperative endoscopic video data.

MoS-based nanocomposites and aerogels for antibiotic pollutants removal from wastewater by photocatalytic degradation process: A review.

Photocatalytic technologies based on molybdenum disulfide (MoS) catalysts are effective, eco-friendly, and promising for antibiotic pollutants treatment. The technologies used by MoS-based nanocomposites and aerogels for efficient degradation of antibiotics are reviewed in detail for the first time in this paper. The fundamental aspects of MoS were comprehensively scrutinized, encompassing crystal structure, optical properties, and photocatalytic principle.

Effects of microcystin-LR on purification efficiency of simulating drinking water source by Hydrocharis dubia (Bl.) backer.

The safety of drinking water source directly affects human health. Microcystin-LR (MC-LR), a toxic and common pollutant in drinking water source, is released by algae and can impede the in-situ remediation effect of aquatic plant. Finding out the effect mechanism of MC-LR on the purification of drinking water by aquatic plant is the key to its application.

Projection of land susceptibility to subsidence hazard in China using an interpretable CNN deep learning model.

Land subsidence is a worldwide geo-environmental hazard. Clarifying the influencing factors of land subsidence hazards susceptibility (LSHS) and their spatial distribution are critical to the prevention and control of subsidence disasters. In this study, we selected natural and anthropogenic features or variables on LSHS and used the interpretable convolutional neural network (CNN) method to successfully construct a LSHS model in China.

A simple control and high accuracy measurement method of open-cell four-electrode conductivity electrode.

The paper presents a simple control and high-accuracy measurement method for a four-electrode conductivity probe based on the principle of the bi-directional voltage pulse. The two-way differential AC (alternating current) pulse voltage signal for the reference resistance and solution resistance is modulated into the single DC stationary voltage response signal to drop the demand for software and hardware and effectively eliminate the influence of excitation voltage pulse amplitude on measurement accuracy, and then the synchronous rectification DC measurement is proposed without any control timing. Meanwhile, the mathematical expression between the single output DC voltage signal and the solution conductivity is given.

Constructing an orderly electron transport channel on boron regulated biomass carbon fiber for selective ROS generation and water decontamination.

Antibiotic pollution has raised widely attention due to the difficult biodegradation and lasting toxicity to public health, metal-free material based heterogeneous catalysis is a highly-promise and eco-friendly technology for organics elimination. Herein, boron doped biomass carbon fiber (B-CF) was synthesized to construct orderly electron transport channels for enhancing catalytic performance and deeply purifying organics polluted water. Integrating systematical quenching experiments and EPR detection, O and O are found to be dominating reactive oxygen species (ROS) for norfloxacin (NOR) degradation rather than ∙OH or SO.

Experimental Investigation on Laser-Induced Electrochemical Silver Plating Technology.

The silver coating is widely used in electronic device manufacturing due to its excellent conductivity and soldering properties. Conventional preparation of local silver coating often uses the preplated silver, mask high-speed silver plating, and deplated silver processes. In this paper, the laser-induced electrodeposition technique is used to perform maskless laser-induced localized electrodeposition on a copper substrate preplated with a layer of silver.

Adhesion-regulating molecule 1 (ADRM1) can be a potential biomarker and target for bladder cancer.

Adhesion-regulating molecule 1 (ADRM1) has been implicated in tumor development, yet its specific role in bladder cancer (BC) remains undefined. This study aimed to elucidate the function of ADRM1 in BC through a combination of bioinformatics analysis and immunohistochemical analysis (IHC). Utilizing R version 3.

The pathological significance and potential mechanism of ARHGEF6 in lung adenocarcinoma.

Background: Emerging evidences suggest that ARHGEF6 is involved in cancers but the exact significance and underlying mechanism are unclear. This study aimed to elucidate the pathological significance and potential mechanism of ARHGEF6 in lung adenocarcinoma (LUAD).

Methods: Bioinformatics and experimental methods were used to analyze the expression, the clinical significance, the cellular function and potential mechanisms of ARHGEF6 in LUAD.

A novel endothelial-related prognostic index by integrating single-cell and bulk RNA sequencing data for patients with kidney renal clear cell carcinoma.

Endothelial cells in the tumor microenvironment play an important role in the development of kidney renal clear cell carcinoma (KIRC). We wanted to further identify the function of endothelial cells in KIRC patients by integrating single-cell and bulk RNA sequencing data. Online databases provide the original data of this study.

Rapid measurement of brown tide algae using Zernike moments and ensemble learning based on excitation-emission matrix fluorescence.

Accurate real-time prediction of microalgae density has great practical significance for taking countermeasures before the advent of Harmful algal blooms (HABs), and the non-destructive and sensitive property of excitation-emission matrix fluorescence (EEMF) spectroscopy makes it applicable to online monitoring and control. In this study, an efficient image preprocessing algorithm based on Zernike moments (ZMs) was proposed to extract compelling features from EEM intensities images. The determination of the highest order of ZMs considered both reconstruction error and computational cost, then the optimal subset of preliminarily extracted 36 ZMs was screened via the BorutaShap algorithm.

Enhanced adsorption of phosphate from pickling wastewater by Fe-N co-pyrolysis biochar: Performance, mechanism and reusability.

A one-step method of preparation using a novel nitrogen (N)-doped Fe-rich biochar (N-CB) resulted in a maximum adsorption capacity (314.52 mg/g) compared with Fe-rich biochar (CB, 104.044 mg/g).

Proanthocyanidins Alleviate Cadmium Stress in Industrial Hemp ( L.).

Industrial hemp ( L.), an annual herbaceous cash crop, is widely used for the remediation of heavy metal-contaminated soils due to its short growth cycle, high tolerance, high biomass, and lack of susceptibility to transfer heavy metals into the human food chain. In this study, a significant increase in proanthocyanidins was found in Yunnan hemp no.

Promotion of higher synthesis temperature for higher-efficient removal of antimonite and antimonate in aqueous solution by iron-loaded porous biochar.

Iron-loaded porous biochar (FPBC) was synthesized by co-pyrolysis method using sawdust and potassium ferrate at 500 (FPBC500) and 800°C (FPBC800), then characterized and applied to eliminate antimonite (Sb(III)) and antimonate (Sb(V)) in aqueous. Due to alkali erosion on feedstock and K/Fe-oxides attacking carbon, FPBC800 obtained a larger specific surface area (SSA) (515.49 m·g) that was 5.

The effects of inorganic anions on degradation kinetics and isotope fractionation during the transformation of tris(2-chloroethyl) phosphate (TCEP) by UV/persulfate.

Tris(2-chloroethyl) phosphate (TCEP), as a typical chlorinated flame retardant, is attracting more attention as a carcinogen. Although persulfate-based oxidation exhibits good performance in removing refractory organic pollutants, the kinetics of persulfate-based remediation are affected by inorganic anions, which causes inaccurate remediation efficiency. This study combines steady-state radical concentration modelling with isotope fractionation to investigate the effects of inorganic anions on TCEP degradation by UV/persulfate (UV/PS).

Rapid in measurements of brown tide algae cell concentrations using fluorescence spectrometry and generalized regression neural network.

The frequent occurrence of brown tide pollution in recent years has brought great losses to the economy of coastal areas. Therefore, accurate and efficient detection of the brown tide algae cell concentration is of great significance to the prevention of marine environmental pollution. In this paper, a combination of three-dimensional fluorescence spectroscopy and generalized regression neural network is used to detect the concentration of Aureococcus anophagefferens (A.

The effect of co-pyrolysis temperature for iron-biochar composites on their adsorption behavior of antimonite and antimonate in aqueous solution.

Iron-biochar is an efficient adsorbent for contaminants, whereas the role of prepared temperature on removal of antimony (Sb) is unacquainted. In this study, the iron-biochar composites (FBC) were fabricated by co-pyrolysis at 500°C and 800°C and applied to remove antimonite (Sb(III)) and antimonate (Sb(V)) in aqueous. The results showed FeO was loaded on biochar prepared at 500°C (FBC500), while FeOOH with zero-valent iron (ZVI) was formed on biochar pyrolyzed at 800°C (FBC800).

MoS nanosheets loaded on collapsed structure zeolite as a hydrophilic and efficient photocatalyst for tetracycline degradation and synergistic mechanism.

In this study, MoS@Z photocatalysts were synthesized by combining ultrasonic and hydrothermal methods, and used for the degradation of tetracycline. The structure characteristics and photocatalytic degradation mechanism of photocatalysts were also systematically investigated. The obtained MoS@Z-5 exhibits the highest photo-degradation efficiency of tetracycline (87.

Highly efficient remediation of groundwater co-contaminated with Cr(VI) and nitrate by using nano-Fe/Pd bimetal-loaded zeolite: Process product and interaction mechanism.

Hexavalent chromium and nitrate co-contaminated groundwater remediation are attracting extensive attention worldwide. However, the transformation pathways of chromium and nitrate and the interplay mechanism between them remain unclear. In this work, zeolite-supported nanoscale zero-valent iron/palladium (Z-Fe/Pd) was synthesized and used for the first time to simultaneously remediate Cr(VI) and nitrate.

The effect of substrates on the removal of low-level vanadium, chromium and cadmium from polluted river water by ecological floating beds.

Ecological floating beds (EFBs) is one of the effective methods lately used to remove heavy metals pollutions in water. However, the role of substrate in EFBs was mainly focused on the study of microorganisms, and the effect of substrates on plants enrichment of heavy metals was rarely investigated. This study aimed to investigate the promotion of different substrates (green zeolite, sepiolite, absorbent paper, and ceramsite) on the removal of multi-heavy metals (V, Cr, and Cd) by Acorus calamus L.

Absorption characteristics of compound heavy metals vanadium, chromium, and cadmium in water by emergent macrophytes and its combinations.

The aim of the present study was to investigate three kinds of emergent macrophytes, i.e., Acorus calamus L.