[Detection the coinfection of Hantavirus and Orientia tsutsugamushi in primary cultured mite cells].

Objective: To investigate whether HV and Ot can coexist in their host (Leptotrombidium scutellare).

Methods: Collecting the separate Leptotrombidium scutellare and the ones from mice in epidemic area. The cells of mites at larva, nymph, and adult stages were cultured and made into smear.

[Study on the coinfection of Hantavirus and Orientia tsutsugamushi in tissue cell culture].

Objective: To investigate the possibility of Hantavirus (HV) and Orientia tsutsugamushi (Ot) coinfection in their hosts.

Methods: HV and Ot were used to infect Vero E6 cells cultured in vitro singly, simultaneously or successively. Genes of HV and Ot were identified in different generation cells with RT-PCR.