Inhibition of AMPK expression in skeletal muscle by systemic inflammation in COPD rats.

Background: Chronic obstructive pulmonary disease (COPD) is a disease characterized by airflow limitation and inflammation. Meanwhile, COPD also is associated with metabolic disorders, such as skeletal muscle weakness. Strikingly, activation of AMP-activated protein kinase (AMPK) exerts critical roles in energy metabolism.

[Iodoacetamide-induced aquaporin 1 expression in fibroblasts is energy-dependent].

Objective: To investigate the impact of energy metabolism at the cellular level on the expression of the water channel protein aquaporin 1 (AQP1).

Methods: Balb/c mouse fibroblasts were incubated with iodoacetamide (IA) in vitro, and the changes in AQP1 expression were detected by immunoblotting and immunohistochemistry at 0, 4, and 6 h.

Results: IA induced the expression of AQP1 at 4 and 6 h accompanied with cell death.