Lactate-upregulated NADPH-dependent NOX4 expression via HCAR1/PI3K pathway contributes to ROS-induced osteoarthritis chondrocyte damage.

Increasing evidence shows that metabolic factors are involved in the pathological process of osteoarthritis (OA). Lactate has been shown to contribute to the onset and progression of diseases. While whether lactate is involved in the pathogenesis of OA through impaired chondrocyte function and its mechanism remains unclear.

Intraperitoneal administration of the globular adiponectin gene ameliorates diabetic nephropathy in Wistar rats.

The present study investigated the potential effects of the long-term expression of exogenous adiponectin (ADPN) on normal and diabetic kidneys. Type 2 diabetes mellitus models were induced by high-lipid and high-sucrose feeding plus intraperitoneal injection of streptozotocin. The recombinant plasmid pIRES2-EGFP-gAd, which is able to co-express globular ADPN (gAd) and enhanced green fluorescent protein (EGFP), was intraperitoneally injected into rat models mediated by Lipofectamine.

Adiponectin inhibits the generation of reactive oxygen species induced by high glucose and promotes endothelial NO synthase formation in human mesangial cells.

The aim of this study was to investigate the effects of adiponectin (ADPN) on high glucose (HG)-induced reactive oxygen species (ROS) and the formation of endothelial nitric oxide (NO) synthase (eNOS) in human glomerular mesangial cells (HMCs), as well as to determine which signaling pathways are modulated by ADPN and the mechanisms involved. HMCs cultured in vitro were randomly divided into 4 groups: the control, HG, HG + globular adiponectin (gAd) and HG + gAd + adenine arabinoside (AraA). The generation of ROS was detected using a fluorescent probe.

[Renoprotective effect of adiponectin through an antioxidant mechanism in streptozotocin- induced diabetic rats].

Objective: To investigate the renoprotective effect of adiponectin in streptozotocin (STz)-induced diabetic rats and explore its association with oxidation stress.

Methods: Type 2 diabetes mellitus was induced in rats by high-lipids and high-sucrose feeding and intraperitoneal STZ injection. The recombinant plasmid pIRES2-EGFP-gAd expressing globular adiponectin was intraperitoneally injected in the rats mediated by liposome.

[Edge detection and modeling in frontal facial contour image for plastic surgery].

Objective: To find a new method for edge detection and modeling in frontal facial contour image.

Methods: Searching circle-based edge detection algorithm was developed on the basis of Sobel edge detector. Apriori knowledge of the facial contour and searching limitations as the minimum curvature radius, concave-convex property, and maximum edge disconnected distance were used to detect the edge of frontal facial contour.