Upregulation of amino acid transporter expression induced by L-leucine availability in L6 myotubes is associated with ATF4 signaling through mTORC1-dependent mechanism.

Objective: Essential amino acids, especially l-leucine, initiate the signaling of the mammalian target of rapamycin complex-1 (mTORC1) and protein synthesis in skeletal muscle. Current information on the relation between amino acid transporter mechanisms and mTORC1 signaling is sparse. The objectives of this study were to determine whether an increase in leucine availability upregulates the gene transcription and translation of amino acid transporters and other amino acid members in an mTORC1-dependent pathway that control amino acid use (general control non-repressed-2 and activating transcription factor-4) and to measure the factors related to protein synthesis and proteolysis.