[Genotype 1 JEV was isolated again from Liaoning Province, China, 2007].

Objective: To isolate Japanese encephalitis virus (JEV) from mosquitoes collected in Liaoning province and analysis the genotype of new isolated JEV strains and the characters of nucleotide and amino acid in the E gene.

Methods: Collected mosquitoes in Dandong Liaoning Province in August, 2006. Virus isolation was using issue culture cells.