[Preparation, characterization and preliminary application of monoclonal antibody against human mu chain].

Aim: To prepare monoclonal antibody (mAb) against human mu chain with high titer and establish a capture ELISA for early serological diagnosis of infectious diseases.

Methods: BALB/c mice were immunized with human IgM. Hybridoma cell line which could stably secret the mAb to human IgM was established by routine cell fusion technique.

[Preparation of specific monoclonal antibody against nucleocapsid protein of SARS coronavirus].

Aim: To express nucleocapsid(N) protein of SARS coronavirus and produce monoclonal antibody(mAb) to N protein.

Methods: N protein gene was amplified by RT-PCR. After being confirmed by DNA sequencing, the gene was subcloned into prokaryotic expression vector.

[Analysis on patial amino acid sequence of a novel glycoprotein binding to antiherpes simplex virus monoclonal antibody CHA9].

Aim: To acquire the characteristic amino acid sequence of a novel glycoprotein of herpes simplex virus (HSV), so as to localize gene encoding the novel glycoprotein accurately.

Methods: A 12-mer phage peptide library was screened for 3 rounds by using biotinylated mAb CHA9 against new glycoprotein g30k of HSV-2. Positive phage clones were detected by ELISA.

[Expression of Japanese encephalitis virus E protein in methylotrophic yeast Pichia pastoris].

Aim: To express Japanese encephalitis virus (JEV) E protein in methylotrophic yeast Pichia pastoris.

Methods: The gene coding for JEV E protein was sub-cloned into vector pPIC9k. The constructed plasmid was transformed into yeast SMD1168 by electroporation.