[Analysis of methanogenic community of anaerobic granular sludge based on mcrA gene].

The methanogenic community in anaerobic granular sludge from a full-scale UASB treating avernectin wastewater was analyzed based on mcrA gene, compared to 16S rRNA gene. The results indicated that the diversity indices of methanogenic community, including Shannon diversity index, Margalef richness index and Berger-Parker dominance index, were no difference between mcrA gene-based and 16S rRNA gene-based PCR products analysis by DGGE, although their DGGE band patterns were different, implying that the diversity analysis of methanogenic community based on mcrA genes was consistent with 16S rRNA gene. The phylogenetic analysis of dominant methanogenic populations based on these two target genes also showed resemble and Methanobacteriales and Methanosarcinales were determined to be the main orders of methanogenic populations in anaerobic granular sludge.

[Bioaugmented treatment of atrazine by genetically engineered microorganism in different bioreactors].

Removal of atrazine was investigated when genetically engineered microorganism (GEM) was inoculated into membrane bioreactor (MBR) and hybrid bioreactor for bioaugmentation. The performances of atrazine removal in two bioreactors were explored. The variations of GEM density and atzA gene abundance in two bioreactors were also determined.

[Analysis of methanogenic community of anaerobic granular sludge in a full-scale UASB treating avermectin wastewater].

Methanogens is considered to be important functional microbial population in anaerobic granular sludge. Fluorescence in situ hybridization was used to analyze methanogens of anaerobic granular sludge in a full-scale UASB treating avermectin wastewater. The results indicated that the distribution forms of methanogens, Methanobacteriales and Methanosarcinales were identical on the surface and inner face of granular sludge samples in different formation stages (with different diameters) , although the relative abundances of these methanogens were different.