Epigenetic network of EZH2/SFRP1/Wnt in the epithelial-mesenchymal transition of laryngeal carcinoma cells.

Enhancer of Zeste Homologue 2 (EZH2) as a histone methyltransferase epigenetically regulates laryngeal carcinoma (LGC) progression. The present study sought to explore the role and mechanism of EZH2 in the epithelial-mesenchymal transition (EMT) of LGC cells. Expressions of EZH2, secreted frizzled-related protein 1 (SFRP1), and trimethylation of lysine 27 on histone H3 (H3K27me3) in LGC tissues or cells were detected via reverse transcription quantitative polymerase chain reaction (qRT-PCR) and western blotting.

Pyrroline-5-carboxylate synthase senses cellular stress and modulates metabolism by regulating mitochondrial respiration.

Pyrroline-5-carboxylate synthase (P5CS) catalyzes the synthesis of pyrroline-5-carboxylate (P5C), a key precursor for the synthesis of proline and ornithine. P5CS malfunction leads to multiple human diseases; however, the molecular mechanism underlying these diseases is unknown. We found that P5CS localizes in mitochondria in rod- and ring-like patterns but diffuses inside the mitochondria upon cellular starvation or exposure to oxidizing agents.

Analyses and treatments of postoperative nasal complications after endonasal transsphenoidal resection of pituitary neoplasms.

In this study, we analyze and discuss the treatments of postoperative nasal complications after endonasal transsphenoidal resection of pituitary neoplasms (PNs). We performed 129 endonasal transsphenoidal resections of PNs and analyzed and treated cases with nasal complications. After endonasal transsphenoidal resection of PNs, there were 26 cases of postoperative nasal complications (20.

[Quantifying the influence of different matrices on Pb accumulation in the soil from Nanjing and suspended matter from the lower of the Yangtze River with Pb isotopic technique].

Lead pollution and its geochemical behavior have an influence on ecological environment and human health. It is of significance to study the characteristics and mechanism of Pb accumulation and to quantify the contributions of different environmental matrices to Pb accumulation in soils and river suspended matter in the representative fluvial delta area, such as the lower of the Yangtze River. The results showed that lead was enriched in the urban topsoil in Nanjing and the suspended matter in the lower of the Yangtze River.

[The effect of nasal irrigation in the treatment of allergic rhinitis].

Objective: To compare the symptoms and lower airway inflammatory factors of patients with allergic rhinitis (AR), and to observe the effect of nasal irrigation in the treatment of allergic rhinitis.

Method: Sixty-one cases diagnosed as AR after skin prick test (SPT)were selected and randomly divided into three groups: 17 patients in group A used nasal steroid spray; 21 cases in group B used nasal irrigation; 23 patients in group C combined ir rigation with nasal steroid. Before and after 3 months treatment, nasal visual analogue scale (VAS) score, rhino conjunctivtis quality of life questionnaire (RQLQ) score, fractional exhaled nitric oxide (FENO) values were observed and compared in each group.

Isolated frontal sinusitis treated using an anterior-to-ethmoidal bulla surgical approach.

Traditional frontal sinus surgery is associated with a significant trauma. Herein, we have discussed the feasibility, technique, and efficacy of a minimally invasive anterior-to-ethmoidal bulla surgical approach performed under nasal endoscopy to treat isolated frontal sinusitis. Fifteen patients with isolated frontal sinusitis underwent the anterior-to-ethmoidal bulla surgical procedure under general anesthesia.

Repair of ear cartilage defects with allogenic bone marrow mesenchymal stem cells in rabbits.

The study aims to investigate the feasibility of repairing cartilaginous defects with chondrocytes induced from allogenic bone marrow mesenchymal stem cells (BMMSC) in rabbits' ear. BMMSCs were isolated and purified from New Zealand rabbits, in vitro amplified, and cultured in chondrocyte induction medium in order to acquire chondrocytes. After 3 weeks of induction, their phenotypes were confirmed as chondrocytes, then they were implanted onto novel polymeric scaffolds made from Poly (dl-lactide-co-glycolide) (PLGA) embedded with chitosan nonwoven cloth.

GSTM1 null polymorphisms is associated with laryngeal cancer risk: a meta-analysis.

Many studies have examined the association between the GSTM1 (null or non-null genotype) polymorphism and laryngeal cancer risk in various populations, but their results have been inconsistent. To assess this relationship more precisely, a meta-analysis was performed. PubMed was searched for case-control studies published up to December 2013.

Transplantation of fetal instead of adult fibroblasts reduces the probability of ectopic ossification during tendon repair.

Although cell transplantation therapy can effectively promote functional tendon repair, occasional ectopic ossification during tendon regeneration undermines its efficacy. The effect of transplanted cell types on ectopic ossification has not yet been systematically evaluated. This study compared the rate of ectopic ossification during tendon repair upon transplantation with mouse fetal fibroblasts (FFs) and their adult counterparts (adult fibroblasts [AFs]).

The effect of decellularized matrices on human tendon stem/progenitor cell differentiation and tendon repair.

It is reported that decellularized collagen matrices derived from dermal skin and bone have been clinically used for tendon repair. However, the varying biological and physical properties of matrices originating from different tissues may influence the differentiation of tendon stem cells, which has not been systematically evaluated. In this study, the effects of collagenous matrices derived from different tissues (tendon, bone and dermis) on the cell differentiation of human tendon stem/progenitor cells (hTSPCs) were investigated, in the context of tendon repair.

[Quantitative prediction of soil salinity content with visible-near infrared hyper-spectra in northeast China].

Studying the spectral property of salinized soil is an important work, for it is the base of monitoring soil salinization by remote sense. To investigate the spectral property of salinized soil and the relationship between the soil salinity and the hyperspectral data, the field soil samples were collected in the region of Northeast China and then reflectance spectra were measured. The partial least squares regression (PLSR) model was established based on the statistical analysis of the soil salinity content and the reflectance of hyperspectra.

Expression of endothelin receptor subtypes in the spiral ganglion neurons of the guinea pig.

Objective: Endothelin 1 has many biological activities including regulating the functions of auditory system. The present study aimed to investigate the expression of the endothelin receptors in spiral ganglion neurons and its significance in the auditory transmission.

Methods: The cochleae of healthy guinea pigs were fixed, decalcified, embedded in paraffin and serially sectioned.

4,4'-Di-3-pyridyl-2,2'-dithio-dipyrimidine.

The asymmetric unit of the title compound, C(18)H(12)N(6)S(2), contains one half-mol-ecule situated on a twofold rotational axis that passes through the mid-point of the S-S bond. In the mol-ecule, the C-S-S-C torsion angle is 81.33 (7)°.

{Bis[4-(2-pyrid-yl)pyrimidin-2-yl]sulfane}dichloridocobalt(II).

The asymmetric unit of the title compound, [CoCl(2)(C(18)H(12)N(6)S)], contains one half-mol-ecule situated on a twofold rotation axis which passes through the Co and S atoms. The metal centre is in a distorted octahedral CoCl(2)N(4) coordination with the Cl atoms in the axial positions. In the crystal structure, inter-molecular C-H⋯Cl inter-actions help to establish the packing.

[Characteristics of heavy metals in sediments from different sources and their ecological risks in the lower reaches of the Yangtze River].

Characteristics of heavy metals in sediments from different sources are significant varied,which impact on the degree of damage for aquatic system. Samples from municipal, mining, industrial and port sources in the lower reaches of the Yangtze River were analyzed for major elements and Cu, Zn, Pb, Cd, Cr by ICP-AES and AAS. The results showed that the concentrations were moderately and relatively uniform for heavy metals in the municipal source, Cu, Pb were dominated for the mine source.

(S)-1-[3,5-Bis-(trifluoro-meth-yl)phen-yl]-N-methylethyl-amine-(R)-2-hydroxy-butane-dioic acid (1/1).

In the title compound, C(11)H(11)F(6)N·C(4)H(6)O(5), a key inter-mediate in the synthesis of the NK1 receptor antagonist of casopitant, the F atoms of the trifluoro-methyl groups are disordered over two sites with equal occupancies. In the crystal, the components are linked by bifurcated N-H⋯(O,O) hydrogen bonds.

3-{[4-(4-Pyrid-yl)pyrimidin-2-yl]sulfanylmeth-yl}benzoic acid.

The title compound, C(17)H(13)N(3)O(2)S, was prepared by reaction of 4-(4-pyrid-yl)pyrimidine-2-thiol with 3-(bromo-meth-yl)benzoic acid under basic conditions. Each pair of mol-ecules is mutually linked via O-H⋯N hydrogen bonds, forming a dimer. The packing of the dimers is stablized by C-H⋯π inter-actions involving the methyl-ene unit of the -CH(2)S- linkage and benzene rings.

1,3,4,6-Tetra-chloro-7,7-bis-(4-chloro-phen-yl)bicyclo-[4.2.0]oct-3-ene-2,5-dione.

The title compound, C(20)H(10)Cl(6)O(2), a quinone derivative, was obtained by the irradiation of 2,3,5,6-tetra-chloro-benzo-quinone and 4,4'-(ethene-1,1-di-yl)bis-(chloro-benzene). The six- and four-membered rings are fused in a cis configuration. The dihedral angle between them is 53.

Relatively light general anesthesia is more effective than fluid expansion in reducing the severity of epinephrine-induced hypotension during functional endoscopic sinus surgery.

Background: Epinephrine infiltration of the nasal mucosa causes hypotension during functional endoscopic sinus surgery (FESS) under general anesthesia. A prospective randomized-controlled study was designed to determine whether relatively light general anesthesia is superior to fluid expansion in reducing epinephrine-induced hypotension during FESS.

Methods: Ninety patients undergoing elective FESS under general anesthesia were randomly assigned to three groups with 30 patients in each.

Reflectance spectroscopy study of Cd contamination in the sediments of the Changjiang River, China.

Sediments of the Changjiang River have been found in recent studies to be enriched in cadmium (Cd). The possibility and mechanisms for evaluating total Cd concentration and its binding form using reflectance spectroscopy within the visible-near-infrared (VNIR) region (400-2500 nm) have been investigated. Bottom sediments (69 samples) in the lower reaches of the river were collected for chemical analyses and spectral measurements.

Expressions of cytokines and chemokines in the dorsal motor nucleus of the vagus nerve after right vagotomy.

The aim of this study was to investigate the expression of cytokines, tumor necrosis factor alpha (TNF-alpha), interleukin-1 beta (IL-1beta), interleukin-6 (IL-6) and transforming growth factor-beta 1 (TGF-beta1) and chemokines, fractalkine, monocyte chemoattractant protein 1 (MCP-1) and stromal cell-derived factor 1 (SDF-1) in the dorsal motor nucleus of the vagus nerve (DMV) after right vagotomy. Results showed that the immunoreactivities of IL-1beta, IL-6, TGF-beta1, fractalkine and MCP-1 were upregulated in the DMV at 14 days and the upregulation persisted at least until 28 days following right vagotomy. Quantification analysis revealed significant increases in the number of their immunopositive cells in the right DMV at 14 and 28 days after right vagotomy.

Feasibility of reflectance spectroscopy for the assessment of soil mercury contamination.

Conventional methods for investigating soil Hg contamination based on raster sampling and chemical analysis are time-consuming and relatively expensive. The objective of this study was to develop a rapid method for investigating Hg concentration in suburban agricultural soils of the Nanjing region using reflectance spectra within the visible-near-infrared (VNIR) region. Several spectral pretreatments (absorbance, Kubelka-Munk transformations and their derivatives) were applied to the reflectance spectra to optimize the accuracy of prediction.

Interactions of chemokines and chemokine receptors mediate the migration of mesenchymal stem cells to the impaired site in the brain after hypoglossal nerve injury.

Mesenchymal stem cells (MSCs), cultured ex vivo, recently were shown to be able to migrate into sites of brain injuries when transplanted systemically or locally, suggesting that MSCs possess migratory capacity. However, the mechanisms underlying the migration of these cells remain unclear. In this study, we examined the role of some chemokines and their receptors in the trafficking of rat MSCs (rMSCs) in a rat model of left hypoglossal nerve injury.

Expression of chemokine receptors CXCR4, CCR2, CCR5 and CX3CR1 in neural progenitor cells isolated from the subventricular zone of the adult rat brain.

We have studied the expression of chemokine receptors CXCR4, CCR2, CCR5, and CX3CR1 at the mRNA and protein levels in adult neural progenitor cells (NPCs) in neurosphere cultures using RT-PCR and immunocytochemistry methods. NPCs were isolated from the subventricular zone of adult rat brain and propagated in vitro as neurospheres. The neurospheres showed immunoactivity of nestin, an intermediate filament marker for NPCs.