[Protective effect and mechanism of curcumin on ActD/TNF-α-induced synergistically apoptosis in PC12 cells].

Aim: To investigate the protective effect and mechanism of curcumin against ActD/TNF-α-induced synergistically apoptosis in PC12 cells.

Methods: MTT assay was used to evaluate the optimal concentration of drugs; Hoechst 33258 fluorescent staining to observe apoptosis of PC12 cells, JC-1 was used to detect the mitochondrial membrane potential (MMP), real-time PCR was used to detect the expression of two apoptotic genes: Bcl-1 and Bax.

Results: ActD/TNF-α can synergistically reduce viability of PC12 cells(P<0.