[Effect of rosiglitazone on the expression of T-bet/GATA-3 in T lymphocytes in patients with acute asthma].

Objective: To investigate the effect of rosiglitazone on the expression of T-bet/GATA-3 in peripheral blood T lymphocytes and cytokine derived from Th1/Th2 in patients with acute asthma and to compare its mechanism with that of dexamethasone (DXM).

Methods: Peripheral blood T lymphocytes from 10 patients with acute asthma (A group, A) and 10 healthy volunteers (B group, B) were isolated, purificated and cultured, and were divided into 2 groups (3 h and 24 h) based on the treatment time, and each group was further divided, on the basis of the treatment given, into 3 sub-groups respectively: control group (A(1) and B(1)), rosiglitazone treated group (A(2) and B(2)) and DXM treated group (A(3) and B(3)). The levels of IFN-gamma and IL-4 in the culture supernatant were detected by Enzyme-linked immunoadsorbent assay (ELISA) and the expression levels of T-bet mRNA/GATA-3 mRNA in T lymphocytes was detected by Reverse Transcription-Polymerase Chain Reaction (RT-PCR).

Effect of Yangqixue Qufengshi Recipe on rheumatoid arthritis model mice under different genetic backgrounds.

Objective: To study the effect of Yangqixue Qufengshi Recipe (YQXQFS) on rheumatoid arthritis (RA) model mice under different genetic backgrounds.

Methods: Collagen Induced Arthritis (CIA) were established on HLA-DR4 transgenic (TG) mice and non-transgenic (NTG) mice, which partly were raised with YQXQFS, and the onset day of CIA, the level of type II collagen (CII)-reactive antibodies and the pathological scores of CIA were assessed.

Results: Under HLA-DR4 TG background (compared with NTG mice), the earlier onset day of CIA (11.

[Influence of peroxisome proliferator-activated receptor gamma on airway inflammation of guinea pigs with asthma].

Objective: To investigate the influence and mechanism of peroxisome proliferator-activated receptor gamma (PPAR-gamma) and its ligand agonist rosiglitazone on airway inflammation of guinea pigs with asthma.

Methods: 35 guinea pigs were divided into 5 groups by random number meter: a control group (A group), an asthma group (B group), a dexamethasone (DXM) group (C group), a rosiglitazone group (D group), and a rosiglitazone + 1/2 DXM group (E group), with 7 guinea pigs in each. Bronchoalveolar lavage (BAL) cell count and differential was studied, and the pathologic alteration of the bronchi and the lung tissue was observed.

[Expression of Caspase-3 and Bcl-2 in bladder transitional carcinoma and their significance].

Background & Objective: Tumor growth was the result of cell excessive proliferation and lack of apoptosis. Apoptosis has been considered to be closely associated with Caspase-3 and Bcl-2. This study was designed to investigate the expression of Caspase-3 and Bcl-2 to evaluate their effects on the tumorigenesis and progression in bladder transitional carcinoma (BTCC).

[Nuclear factor-kappa B activity at the early stage of brain ischemia and reperfusion in mice].

Objective: To observe the activity of nuclear factor-kappa B(NF-kappa B) during reperfusion after temporary brain ischemia and to evaluate its effect.

Methods: The brain ischemia-reperfusion(I/R) model of mice was established with ligating bilateral common carotid arteries and bleeding for 0.3 ml in the tail.