Lysine-specific demethylase 1 in breast cancer cells contributes to the production of endogenous formaldehyde in the metastatic bone cancer pain model of rats.

Background: Bone cancer pain seriously affects the quality of life of cancer patients. Our previous study found that endogenous formaldehyde was produced by cancer cells metastasized into bone marrows and played an important role in bone cancer pain. However, the mechanism of production of this endogenous formaldehyde by metastatic cancer cells was unknown in bone cancer pain rats.

[Study on functions and mechanism of curcumin in inducing gastric carcinoma BGC apoptosis].

Aim: discuss the biological function and regulation mechanism of curcumin in promoting human gastric carcinoma BGC-823 apoptosis.

Methods: Conventional in virto culture in logarithmic phase gastric carcinoma BGC-823 cells; cells are divided into four groups: control group, low treatment group, middle treatment group and high treatment group, with curcumin concentration being 0 mg/L, 5 mg/L, 10 mg/L, and 20 mg/L, respectively. 24 hours after curcumin is treated, cell proliferation level and apoptosis rate are measured with MTT colorimetry and flow cytometry, Bax, Bcl-2 protein expression is measured with immunohistochemistry; mRNA of Caspase-3 is tested by means of PCR.

[Potential application of human amniotic mesenchymal cells for seeding cells in bone tissue engineering].

Objective: To investigate the potential of human amniotic mesenchymal cells (hAMC) serving as seeding cells in bone tissue engineering.

Methods: hAMC were isolated and cultured. The third passage of hAMC was cultured in osteogenic induced media [DMEM supplemented with 10% (v/v) FBS, 0.

Histological and ultrastructural changes induced by selenium in early experimental gastric carcinogenesis.

Aim: To investigate the effect and significance of selenium in early experimental gastric carcinogenesis.

Methods: Weaning male Wistar rats were divided randomly into normal control group, experiment control group, low selenium (2 mg/L) group and high selenium (4 mg/L) group. Wistar rat gastric carcinogenesis was induced by N-methyl-N-nitro-N-nitroso guanidine (MNNG) (20 mg/kg) gavage daily for 10 d.