MicroRNA-298 determines the radio-resistance of colorectal cancer cells by directly targeting human dual-specificity tyrosine(Y)-regulated kinase 1A.

Background: Radiotherapy stands as a promising therapeutic modality for colorectal cancer (CRC); yet, the formidable challenge posed by radio-resistance significantly undermines its efficacy in achieving CRC remission.

Aim: To elucidate the role played by microRNA-298 (miR-298) in CRC radio-resistance.

Methods: To establish a radio-resistant CRC cell line, HT-29 cells underwent exposure to 5 gray ionizing radiation that was followed by a 7-d recovery period.

[Correlations of c-erb-B2, EGFR, and TGF-alpha expression to recurrence of Dukes'A and B colorectal carcinoma].

Background & Objective: Currently, pathologic stage is the main prognostic indicator of colorectal carcinoma, but the current staging system is insufficient to predict the risk of recurrence or the need for adjuvant treatment for the patients with Dukes'A and B disease. Biologic prognostic markers may supplement the staging system and provide a basis for the decision of therapeutic strategies according to individual tumor biology. This study was to investigate the correlations of c-erbB-2, epithelial growth factor receptor (EGFR), and transforming growth factor-alpha (TGF-alpha) expression to recurrence of Dukes'A and B colorectal carcinoma.

[Experimental study on antitumor effects of Shen Qi Jin Kang (SQJK)].

Background & Objective: SHEN QI JIN KANG (SQJK) capsule is a complex preparation, consisting of effective components extracted from radix astragali, ginseng, curcuma, etc. It has been demonstrated to be able to decrease tumor volume, increase life quality and prolong survival time in clinic application. The study was to investigate the antitumor effects of SQJK capsule in vivo and in vitro, and further explore the possible mechanisms.

[Expression of P33ING1, P53 and their relationship with apoptosis in anal canal carcinoma].

Objective: To explore the expressions of P33ING1, P53 and their relationships with apoptosis in anal canal carcinoma (ACC).

Methods: The expressions of P33ING1, P53 proteins were measured by immunohistochemistry method (SP method), and apoptosis was detected in 42 cases with ACC, 36 cases with anal canal adenoma (ACA) or anal canal papilloma (ACP), and 40 cases with paraanal inflammatory mass(PAIM).

Results: The positive expression rates of P33ING1 and P53 proteins were 40.

Outcome of surgical management of the bladder in advanced colorectal cancer.

Background: To evaluate morbidity, mortality, and long-term survival in patients undergoing partial or total cystectomy during en bloc resection for locally advanced colorectal cancer.

Methods: This study retrospectively evaluated the outcome of combined bladder resection for colorectal cancer in our department.

Results: Patients (n=33) with colorectal tumors adherent to the bladder were followed.

Genetic alterations and expression of inhibitor of growth 1 in human sporadic colorectal cancer.

Aim: To explore the effect and significance of inhibitor of growth 1 (ING1) gene in carcinogenesis and progression of human sporadic colorectal cancer.

Methods: mRNA expression, mutation, and loss of heterozygosity (LOH) of ING1 gene in 35 specimens of sporadic colorectal cancer tissues and the matched normal mucous membrane tissues were detected by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR), PCR-single strain conformation polymorphism (PCR-SSCP) and PCR-simple sequence length polymorphism (PCR-SSLP) using microsatellite markers, respectively.

Results: The average ratios of light intensities of p33(ING1b) and p47(ING1a) mRNA expression in the cancerous tissues were significantly lower than those in normal tissues.

[Clinical analysis of anorectal malignant melanoma].

Objective: To summarize the clinicopathological characteristics of primary anorectal malignant melanoma (AMM).

Methods: Clinical data of nine patients with AMM were reviewed retrospectively from January 1999 to March 2005.

Results: Anorectal malignant melanoma had a female predominance.

[Multivariate regression analysis of recurrence following curative surgery for colorectal cancer].

Background & Objective: Recurrence is a very important prognostic factor for colorectal cancer patients after operation. The selection of patients for individualized follow-up and adjuvant therapy after curative resection of colorectal carcinoma depends on prognostic factors for recurrence. The objective of this study was to investigate the clinicopathologic factors related to recurrence following curative surgery for colorectal cancer.