Exploring the Protective Effect and Mechanism of on Sodium Selenite-Induced Cataract in Rats by Network Pharmacology, Molecular Docking, and Experimental Validation.

Objective: has a long history of utilization by humans to treat ophthalmic diseases. Although study revealed that it can be used for treating cataract, the bioactive components and the mechanism of efficacy remained unclear. This study aims to discover the bioactive components and mode of efficacy of in cataract treatment.

Endothelium-derived semaphorin 3G attenuates ischemic retinopathy by coordinating β-catenin-dependent vascular remodeling.

Abnormal angiogenesis and regression of the diseased retinal vasculature are key processes associated with ischemic retinopathies, but the underlying mechanisms that regulate vascular remodeling remain poorly understood. Here, we confirmed the specific expression of semaphorin 3G (Sema3G) in retinal endothelial cells (ECs), which was required for vascular remodeling and the amelioration of ischemic retinopathy. We found that Sema3G was elevated in the vitreous fluid of patients with proliferative diabetic retinopathy (PDR) and in the neovascularization regression phase of oxygen-induced retinopathy (OIR).

CRISPR-Sunspot: Imaging of endogenous low-abundance RNA at the single-molecule level in live cells.

CRISPR/Cas-based mRNA imaging has been developed to labeling of high-abundance mRNAs. A lack of non-genetically encoded mRNA-tagged imaging tools has limited our ability to explore the functional distributions of endogenous low-abundance mRNAs in cells. Here, we developed a CRISPR-Sunspot method based on the SunTag signal amplification system that allows efficient imaging of low-abundance mRNAs with CRISPR/Cas9.

Lewis Acid Dominant Windmill-Shaped V Clusters: A Bifunctional Heterogeneous Catalyst for CO Cycloaddition and Oxidation of Sulfides.

Carbon dioxide (CO) and sulfides in gasoline are the main causes of air pollution. Considerable attention has been devoted to solving the problems, and the catalytic reaction seems to be a good choice. Owing to the high density of Lewis acid (LA) active sites and large numbers of open methoxide groups, polyoxovanadates (POVs) are an undisputed option as a heterogeneous catalyst for the CO cycloaddition reaction and catalytic oxidation of sulfides.