F11R RNA trinucleotide over-edited by ADAR in gastric and colorectal cancers: Cross-cohort validation, gene expression regulation, and diagnostic significance.

The F11 receptor (F11R) gene encoding junctional adhesion molecule A has been associated with gastric cancer (GC) and colorectal cancer (CRC), in which its role and regulation remain to be further elucidated. Recently F11R was also identified as a potential target of adenosine-to-inosine (A-to-I) mediated by the adenosine deaminases acting on RNA (ADARs). Herein, using RNA-Seq and experimental validation, our current study revealed an F11R RNA trinucleotide over-edited by ADAR, with its regulation of gene expression and clinical significance in four GC and three CRC cohorts.

Rapid and efficient isolation platform for plasma extracellular vesicles: EV-FISHER.

Extracellular vesicles (EVs) have found diverse applications in clinical theranostics. However, the current techniques to isolate plasma EVs suffer from burdensome procedures and limited yield. Herein, we report a rapid and efficient EV isolation platform, namely, EV-FISHER, constructed from the metal-organic framework featuring cleavable lipid probes (PO -spacer-DNA-cholesterol, PSDC).

[Effects of water and nitrogen regulation on the yield and water and nitrogen use efficiency of cotton in south Xinjiang, Northwest China under plastic mulched drip irrigation].

A field experiment with three irrigation amounts and five nitrogen application levels was conducted to investigate the effects of water and nitrogen regulation on the growth characteristics, yield component factors, and water and nitrogen use efficiency of cotton in south Xinjiang under mulched drip irrigation. With the increasing amount of irrigation, the plant height, leaf number on main stem, boll number, LAI, and dry matter accumulation in leaf and stem improved significantly, but the root growth was restrained. As compared with low and high irrigation amounts (4950 and 6750 mm x hm(-2), respectively), medium irrigation amount (5850 mm x hm(-2)) increased the available bolls per plant and the single boll mass averagely by 0.

Identification of a novel infection-enhancing epitope on dengue prM using a dengue cross-reacting monoclonal antibody.

Background: Dengue virus (DENV) infection is the most important arthropod- borne viral disease in human, but antiviral therapy and approved vaccines remain unavailable due to antibody-dependent enhancement (ADE) phenomenon. Many studies showed that pre-membrane (prM)-specific antibodies do not efficiently neutralize DENV infection but potently promote ADE infection. However, most of the binding epitopes of these antibodies remain unknown.