Root extractive from benefits in wound healing of anal fistula through up-regulation of collagen genes in human skin fibroblasts.

Wound healing is the main problem in the therapy of anal fistula (AF). root has been traditionally used as an agent to soak sutures in operation of AF patients, but its function in wound healing remains largely unclear. The aim of the present study was to illuminate mechanisms of root treatment on AF.

Cancer Cell Membrane-Coated Upconversion Nanoprobes for Highly Specific Tumor Imaging.

Cancer cell membrane-coated upconversion nanoprobes (CC-UCNPs) with immune escape and homologous targeting capabilities are used for highly specific tumor imaging. The combination of UCNPs with biomimetic cancer cell membranes embodies a novel materials design strategy and presents a compelling class of advanced materials.

Synthetic nanoparticles camouflaged with biomimetic erythrocyte membranes for reduced reticuloendothelial system uptake.

Suppression of the reticuloendothelial system (RES) uptake is one of the most challenging tasks in nanomedicine. Coating stratagems using polymers, such as poly(ethylene glycol) (PEG), have led to great success in this respect. Nevertheless, recent observations of immunological response toward these synthetic polymers have triggered a search for better alternatives.

Red Blood Cell Membrane as a Biomimetic Nanocoating for Prolonged Circulation Time and Reduced Accelerated Blood Clearance.

For decades, poly(ethylene glycol) (PEG) has been widely incorporated into nanoparticles for evading immune clearance and improving the systematic circulation time. However, recent studies have reported a phenomenon known as "accelerated blood clearance (ABC)" where a second dose of PEGylated nanomaterials is rapidly cleared when given several days after the first dose. Herein, we demonstrate that natural red blood cell (RBC) membrane is a superior alternative to PEG.

Approaches to the evaluation of malaria elimination at county level: case study in the Yangtze River Delta region.

As the progress on transition from malaria control to malaria elimination in the People's Republic of China (P.R. China), four counties/districts, namely Zhabei District and Songjiang District of Shanghai municipality, and Anji County and Haiyan County of Zhejiang Province, representatives of the Yangtze River Delta region, were included in the pilot project of the national malaria elimination programme in P.

Core-shell supramolecular gelatin nanoparticles for adaptive and "on-demand" antibiotic delivery.

The treatment of bacterial infection is one of the most challenging tasks in the biomedical field. Antibiotics were developed over 70 years and are regarded as the most efficient type of drug to treat bacterial infection. However, there is a concern that the overuse of antibiotics can lead to a growing number of multidrug-resistant bacteria.

Supramolecular gelatin nanoparticles as matrix metalloproteinase responsive cancer cell imaging probes.

We demonstrate the on-chip preparation of size-controllable supramolecular gelatin nanoparticles (SGNs) with a quantum dot (QD) payload as matrix metalloproteinase (MMP) responsive cancer cell imaging probes.

[Expression of JAG1 and DLL1 genes in colorectal cancer and its clinical significance].

Objective: To study the expression of JAG1 and DLL1 in colorectal cancer and its clinical significance.

Methods: Patients with colorectal cancer were treated in the Center of Colorectal Surgery of the Third Affiliated Hospital of Nanjing University of TCM were collected prospectively and followed up. A tissue microarray was made and expressions of JAG1 and DLL1 were detected by immunohistochemical staining.

[Expression pattern and clinical significance of hairy enhancer of split 1 in colorectal cancer].

Objective: To explore the roles of hairy enhancer of split 1 (Hes1) gene in colorectal cancer and analyze its clinical significance.

Methods: A total of 146 cases with colorectal cancer at our hospital were collected prospectively and followed up. There were 84 males and 62 females with an average age of (61 ± 11) years old.

Overexpression of the human ZNF300 gene enhances growth and metastasis of cancer cells through activating NF-kB pathway.

Zinc finger proteins (ZNF) play important roles in various physiological processes. Here we report that ZNF300, a novel zinc finger protein, identified specifically in humans, promotes tumour development by modulating the NF-κB pathway. Inflammatory factors were found to induce ZNF300 expression in HeLa cell line, and ZNF300 expression further enhanced NF-κB signalling by activating TRAF2 and physically interacting with IKKβ.

PU.1 can regulate the ZNF300 promoter in APL-derived promyelocytes HL-60.

ZNF300, which plays the role in human embryonic development and some diseases, is a typical KRAB/C2H2 zinc finger gene expressed only in higher mammalians. Our data showed that expression of ZNF300 changed significantly in various leukemia blasts in the bone marrow aspirates of newly diagnosed leukemia patients. To investigate the potential relationship between expression of ZNF300 and the progression of leukemia development and hematopoietic differentiation, we cloned and characterized the putative human ZNF300 gene promoter and identified its transcription start sites (TSSs).

The expression and significance of IDH1 and p53 in osteosarcoma.

Background: To detect the expression of isocitrate dehydrogenase 1 (IDH1) and transformation-related protein 53 (p53) in osteosarcoma and analyze the correlation between them and the clinico-pathological features.

Methods: The expressions of IDH1 and p53 were detected in human osteosarcoma cell lines (MG-63 and U2OS) by immunocytochemistry, Real-time PCR and Western Blotting. The expressions of IDH1 and p53 in formalin-fixed paraffin-embedded tissue sections from 44 osteosarcoma patients were determined by immunohistochemistry, and the correlation between them and clinicopagthological features were analyzed.

[Clinical multi-manifestation, diagnosis and treatment of non-Hodgkin's lymphoma in oral, maxillofacial and cervical regions].

Objective: To investigate the clinical multimanifestation, diagnosis and treatment of non-Hodgkin's lymphoma in oral, maxillofacial and cervical regions.

Methods: 58 cases of non-Hodgkin's lymphoma in oral, maxillofacial and cervical regions were retrospected in this study.

Results: The manifestation of 58 cases was complicated.