Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
December 2009
Aim: Analyze the effect of Tetracycline-controlled system on Hepatitis B virus core promoter (Cp) activity and tissue specificity.
Methods: The 7 Teto sequence was amplified from plasmid pTL-8 using polymerase chain reaction (PCR) and cloned into T vector pMD19-T Simple. After sequencing, the 7 teto sequence was subcloned into upstream of Cp in pGL3-Basic/Cp.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
February 2007
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
April 2005
Objective: To explore the effect of DNA/MVA combined immunization in enhancing antibody response to MSP1.
Methods: DNA vaccine and recombined MVA were constructed based on synthesized MSP1 gene (3D7). BALB/c mice were primed with DNA solely or together with GM-CSF expressing plasmid and then boosted with rMVA/ 190.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
June 2004
Objective: To explore the effect of cytokine encoding plasmids on DNA immunization in mice.
Methods: Prototype DNA vaccine VR1020/E which contain Plasmodium falciparum apical membrane antigen 1 (AMA1) ectodomain was constructed, and eukaryotic expression vectors pcDNA3/GM-CSF, pcDNA3.1(-)/IL-4, pIL-12 and pGM-CSF/pTPA-E were also built.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
April 2004
Objective: To construct tetracycline operator (TetO) modified glycophorin binding protein 130 gene (GBP130) promoter of Plasmodium falciparum and investigate the position effect of insertion of TetO on the promoter activity.
Methods: Cloning of 7-copy of TetO (7cot) sequence into 4 points relative to transcriptional initiation site of GBP130 promoter in pGBPCATdelta2 plasmid (2 upstream and 2 downstream), respectively, produced 4 derivative plasmids, pG/7T (-5), pG/7T (-2), pG/7T (+2) and pG/7T (+5). After transient transfection, the expression level of reporter gene CAT in both pGBPCATdelta2 and its derivative plasmids was detected and analysed by CAT ELISA.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
May 2004
Aim: To construct regulable DNA vaccine against Plasmodium falciparum by using tetracycline(Tet) regulable system.
Methods: Eukaryotic expression vectors pTL-8/apical membrane antigen 1 (AMA-1) (tTA) and pTL-8/AMA-1(rtTA) gene which express trans-activator (tTA) or reverse trans-activator(rtTA), respectively, and AMA-1 gene of Plasmodium falciparum were constructed. BALB/c mice were immunized with these plasmids and doxycycline (dox) was administered to regulate the expression of AMA-1.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
February 2004
Objective: To determine the role of putative apical membrane antigen (AMA)1 domains in inducing protective immunity and to provide basis for selection of vaccine applicable segments.
Methods: Encoding gene segments of AMA1 were amplified and cloned into pET prokaryotic expression vectors. Recombinant proteins were expressed and purified.