Publications by authors named "Jun-Cen Shen"
Article Synopsis
- Researchers developed a new Donor Adapting System (DAS) to enhance the efficiency of homology-directed repair (HDR) in CRISPR gene editing by using a Gal4 DNA binding domain in conjunction with the Cas9 protein.
- The study showed that using a specific length of homologous arms (100-60 bp) in the donor DNA could improve HDR editing efficiency by 2-4 times in a cell line experiment.
- Further optimizations indicated that the choice of fusion components and aptamer designs significantly impact Cas9's performance and HDR success, leading to effective editing at specific genetic sites, which could aid in animal breeding and molecular design.
