Derivation of human-derived iPSC line from a male adolescent with first-episode of sporadic schizophrenia.

Schizophrenia is considered to be a neurodevelopmental disorder with high heritability. In this study, peripheral blood mononuclear cells (PBMCs) were collected from a male adolescent diagnosed with first-episode of sporadic schizophrenia. Induced pluripotent stem cells (iPSCs) were generated by reprogramming using the factors OCT4, SOX2, NANOG, LIN28, c-MYC, KLF4, and SV40LT.

Preparation and application of chitosan/nano-TiO₂/daisy essential oil composite films in the preservation of .

Chitosan, a natural polysaccharide, is widely recognized for its biocompatibility, biodegradability, and film-forming properties, making it an ideal candidate for food preservation applications. This study focuses on the development of chitosan-based nanocomposite films incorporating nano-TiO and Daisy Essential Oil for the preservation of . The composite films were synthesized and characterized using FT-IR, XRD, SEM, TGA, UV-Visible spectroscopy, and contact angle measurements to evaluate their structural, thermal, and physical properties.

Immune repertoire sequencing reveals differences in treatment response to camrelizumab plus platinum-based chemotherapy in advanced ESCC.

This study evaluated the efficacy and safety of camrelizumab combined with platinum-based chemotherapy (taxanes [T] or fluorouracil agents [F] plus platinum [P] drugs) as the first-line treatment in advanced esophageal squamous cell carcinoma (ESCC), using immune repertoire sequencing (IRS) to explore treatment response mechanism. In this multi-center, prospective cohort study, 88 patients received camrelizumab plus TP or FP, achieving a 1-year progression-free survival of 56.8% and overall survival of 68.

Experimental and Model Calculation Research on Shrinkage of Hybrid Fiber-Reinforced Recycled Aggregate Concrete.

Recycled aggregate concrete (RAC), which is made by replacing all natural coarse and fine aggregates with recycled aggregate, plays a significant role in improving the recycling rate of construction materials, reducing carbon emissions from construction, and alleviating ecological degradation issues. However, due to its low strength and significant shrinkage and deformation problems, RAC has limited application. The effort of fiber type, fiber admixture, and fiber hybridization on autogenous shrinkage were studied to improve the structural safety of building materials and broaden the application of RAC.

-Derived Exosome-like Nanovesicles Alleviate Metabolic Dysfunction-Associated Fatty Liver Disease by Promoting Mitophagy and Inhibiting NLRP3 Inflammasome Activation.

Metabolic dysfunction-associated fatty liver disease (MAFLD) affects approximately one-quarter of the world's adult population, and no effective therapeutic drugs are available. is a fungus used as a herb and food nutrient for centuries as well as for MAFLD treatment. Exosome-like nanovesicles have many pharmacological activities; however, studies on the effects of -derived exosome-like nanovesicles (PCELNs) on MAFLD are lacking.

Zinc finger protein ZC3H18 is abnormally expressed in esophageal cancer tissues and facilitates the proliferation of esophageal cancer cells.

Introduction: Esophageal cancer presents significant challenges due to the limited efficacy and severe side effects associated with conventional treatments. The identification of epigenetic regulatory molecules that are aberrantly expressed in tumors is crucial for elucidating the mechanisms underlying the development and progression of esophageal cancer.

Methods: We performed high-throughput methylation level analysis on cancerous and adjacent tissues from 25 patients, identifying the differentially methylated gene utilizing Bismark software and data from TCGA.

Modulation of the human GlyT1 by clinical drugs and cholesterol.

Glycine transporter 1 (GlyT1) is a key player in shaping extracellular glutamatergic signaling processes and holds promise for treating cognitive impairments associated with schizophrenia by inhibiting its activity and thus enhancing the function of NMDA receptors. Despite its significant role in physiological and pharmacology, its modulation mechanism by clinical drugs and internal lipids remains elusive. Here, we determine cryo-EM structures of GlyT1 in its apo state and in complex with clinical trial drugs iclepertin and sarcosine.

Gut microbiota and osteonecrosis: A Mendelian randomization study.

Emerging evidence indicates an association between the gut microbiota and the incidence of osteonecrosis (ON), yet the literature has not adequately addressed whether this relationship is causal. This study uses data from the MiBioGen Consortium and the UK Biobank for Mendelian randomization (MR) analysis to identify pathogenic gut microbial taxa associated with ON. Sensitivity analyses confirmed causal relationships, while reverse MR ruled out reverse causation.

Survival Benefits of Transarterial Chemoembolization Plus Ablation Therapy in Patients With Intermediate or Advanced Hepatocellular Carcinoma: A Propensity Score Matching Study.

Purpose: To evaluate the survival outcomes of patients with intermediate to advanced hepatocellular carcinoma (HCC), patients who underwent transarterial chemoembolization (TACE) alone were compared with those who underwent a combination of TACE and ablation therapy.

Patients And Methods: This study retrospectively evaluated 536 HCC patients in our hospital from July 2016 to November 2022. All patients underwent TACE, with a subset also receiving ablation therapy.

ACA-24-3995.Highlighted: Determination of lopinavir and ritonavir in hERG solution to support in vitro hERG block potency assessment using LC-MS/MS: The challenge of poor drug solubility.

Background: ICH-S7B recommends assessing hERG channel block using patch clamp method to understand the risk of clinical QT prolongation and the associated rare but potentially fatal ventricular tachyarrhythmia, Torsade de Pointes. Drug concentrations exposed to the cells during hERG assays may differ from the intended concentrations due to either human errors or drug properties leading to drug loss. Concentration verification is challenging for drugs with limited solubility, samples may contain solutes and insoluble particles that may solubilize during sample analysis and resulting in inaccurate measurements of cell exposure.

Establishment of a R-Sphere labelled immunoassay platform and its rapid combined quantitative detection of ketamine and morphine in serum.

Background And Aims: Ketamine and morphine are among the most commonly abused drugs. Ketamine is classified as a new psychoactive substance, while morphine represents the opioid class. These two substances may exhibit synergistic or antagonistic effects in their pharmacological actions, and their combined abuse could lead to more severe health risks, such as respiratory depression.

Effectiveness, safety, and patterns of use of camrelizumab in advanced esophageal cancer: an individual patient data pooled analysis of 987 patients from three prospective cohort studies.

Background And Aims: This individual patient data pooled analysis aimed to evaluate the effectiveness, safety, and patterns of use of camrelizumab in a large cohort of advanced esophageal cancer (AEC) patients.

Approach And Results: Adult patients (≥ 18 years) who had received camrelizumab as part of AEC treatment were pooled from three independent, prospective observational cohort studies (NCT04616040, ChiCTR1900027275, and ChiCTR2000039499). The main outcomes were patterns of camrelizumab use, progression-free survival (PFS), overall survival (OS), and safety in the overall population and specific subgroups of underrepresented patients.

Stretchable [2]rotaxane-bridged MXene films applicable for electroluminescent devices.

Titanium carbide (TiCT) MXene has prominent mechanical properties and electrical conductivity. However, fabricating high-performance macroscopic films is challenging, as weak interlayer interactions limit their mechanical performance. Here, we introduce [2]rotaxane, a mechanically interlocked molecule, to enhance MXene films.

Atomically Dispersed Catalytic Platinum Anti-Substitutions in Molybdenum Ditelluride.

Atomic defects, e.g., vacancies, substitutions, and dopants, play crucial roles in determining the functionalities of two-dimensional (2D) materials, including spin glass, single-photon emitters, and energy storage and conversion, due to the introduction of abnormal charge states and noncentrosymmetric distortion.

Nanoscale Resist-Free Patterning of Halogenated Zeolitic Imidazolate Frameworks by Extreme UV Lithography.

Advancements in patterning techniques for metal-organic frameworks (MOFs) are crucial for their integration into microelectronics. However, achieving precise nanoscale control of MOF structures remains challenging. In this work, a resist-free method for patterning MOFs is demonstrated using extreme ultraviolet (EUV) lithography with a resolution of 40 nm.

Ensartinib for advanced or metastatic non-small-cell lung cancer with exon 14 skipping mutations (EMBRACE): a multi-center, single-arm, phase 2 trial.

Background: exon14 skipping mutations (ex14) is an established actionable driver oncogene of non-small-cell lung cancer (NSCLC). While ensartinib is a known second-generation tyrosine kinase inhibitor with primary activity against translocation, it is also classified as a type Ia MET inhibitor. We have previously shown anti-tumor activity against ex14 positive NSCLC both and .

17β-estradiol delays cardiac aging through suppressing the methylation of Beclin1 in a murine model.

Introduction: Cardiac endogenous senescence will gradually change and aggravate with age. Recent research showed that 17β-estradiol (17β-E2), an estrogen with numerous biological activities including the prevention of vascular senescence. However, how 17β-E2 against cardiac aging is still unknown.

Ultra-sparse-view Cone-beam CT Reconstruction Based Strictly-structure-preserved Deep Neural Network in Image-guided Radiation Therapy.

Radiation therapy is regarded as the mainstay treatment for cancer in clinic. Kilovoltage cone-beam CT (CBCT) images have been acquired for most treatment sites as the clinical routine for image-guided radiation therapy (IGRT). However, repeated CBCT scanning brings extra irradiation dose to the patients and decreases clinical efficiency.

Effects of Short-Term Music Therapy on Negative Emotions and Quality of Life in Primiparas.

Objective: This study aimed to explore the effects of short-term music therapy on primiparas' negative emotions and quality of life.

Methods: In this study, 101 pregnant women awaiting delivery in the obstetric department of the hospital from January 2022 to January 2023 were retrospectively analysed. Eleven pregnant women did not fulfil the inclusion criteria, resulting in the inclusion of 90 pregnant women.

Mechanism of Type-II Multiferroicity in Pure and Al-Doped CuFeO_{2}.

Type-II multiferroicity, where electric polarization is induced by specific spin patterns, is crucial in fundamental physics and advanced spintronics. However, the spin model and magnetoelectric coupling mechanisms in prototypical type-II multiferroic CuFeO_{2} and Al-doped CuFeO_{2} remain unclear. Here, by considering both spin and alloy degrees of freedom, we develop a magnetic cluster expansion method, which considers all symmetry allowed interactions.

Similar but different assembly processes of bacterial and micro-eukaryotic communities in an urban river.

Bacteria and micro-eukaryotes play important roles in river ecological systems. The processes that govern bacterial and micro-eukaryotic communities in urban rivers are still uncertain. The spatiotemporal characteristics and assembly processes of bacterial and micro-eukaryotic communities in the Xiangjianghe River were explored using 16 S and 18 S rRNA gene amplicon sequencing in the present study.

Tau pathology is associated with postsynaptic metabotropic glutamate receptor 5 (mGluR5) in early Alzheimer's disease in a sex-specific manner.

Introduction: To investigate the associations of metabotropic glutamate receptor 5 (mGluR5) with tau deposition and cognitive ability in patients with early Alzheimer's disease (AD).

Methods: Twenty-six cognitively impaired (CI) and 14 cognitively unimpaired (CU) individuals underwent mGluR5 positron emission tomography (PET) ([F]PSS232), amyloid PET ([F]florbetapir), and tau PET ([F]MK6240), and neuropsychological assessment. The relationships among mGluR5 availability, tau deposition, and neuropsychological assessment were analyzed using Spearman's correlation and mediation analyses.

Diagnosis of Alzheimer's disease using transfer learning with multi-modal 3D Inception-v4.

Background: Deep learning (DL) technologies are playing increasingly important roles in computer-aided diagnosis in medicine. In this study, we sought to address issues related to the diagnosis of Alzheimer's disease (AD) based on multi-modal features, and introduced a multi-modal three-dimensional Inception-v4 model that employs transfer learning for AD diagnosis based on magnetic resonance imaging (MRI) and clinical score data.

Methods: The multi-modal three-dimensional (3D) Inception-v4 model was first pre-trained using data from the Alzheimer's Disease Neuroimaging Initiative (ADNI) database.

Radiofrequency Ablation Versus Thoracoscopic Sublobar Resection for the Treatment of Pulmonary Ground Glass Nodules: A Retrospective Observational Study.

Purpose: A variety of treatments options for pulmonary ground glass nodules (GGNs) are currently under investigation. This study aimed to retrospectively compare the safety and efficacy of two therapeutic concepts, radiofrequency ablation (RFA) and thoracoscopic sublobar resection, in the treatment of patients with resectable pulmonary GGNs.

Materials And Methods: This bi-center retrospective study included patients with resectable pulmonary GGNs who received either thoracoscopic sublobar resection or percutaneous computed tomography (CT)-guided RFA.

Demystifying death: a qualitative study using the behavior change wheel framework to explore the palliative care education experiences of doctors, nurses, and community residents.

Objective: To explore the palliative care education experiences of doctors, nurses, and community residents.

Methods: A semi-structured interview was conducted with 2 doctors, 8 nurses and 9 community residents in Jinan. Content analysis and behavior change wheel theory were used to analyze the interview content.