Label-Free Quantitative Proteomic Analysis of Three Strains of Viscerotropic Leishmania Isolated from Patients with Different Epidemiological Types of Visceral Leishmaniasis in China.

Background: There are three epidemiological types of visceral leishmaniasis in China, which are caused by Leishmania strains belonging to the L. donovani complex. The mechanisms underlying their differences in the population affected, disease latency, and animal host, etc.

Rapid isolation and determination of bisphenol A in complicated matrices by magnetic molecularly imprinted electrochemical sensing.

Because of its widespread distribution in the environment, bisphenol A (BPA) has become a global concern as an endocrine disruptor and a threat to human health through the food chain. Thus an efficient determination method is urgently needed for monitoring the levels of BPA. Herein, a novel electrochemical technique for the detection of BPA was performed by synchronous extraction and pre-concentration of BPA onto magnetic molecularly imprinted polymer (BMMIP), with subsequent readout on a magneto-actuated glassy carbon electrode (MGCE) by differential pulse voltammetry.

Contributions of the National Institute of Parasitic Diseases to the control of visceral leishmaniasis in China.

Visceral leishmaniasis (VL) caused by Leishmania spp. is an important vector-borne disease prevalent in China. VL was rampant in the vast area of China north of the Yangtze River before the founding of the People's Republic of China in 1949.

[Observation on growth and reproduction of various isolates in different culture media].

Objective: To compare the growth and reproduction of the promastigotes of isolates from various endemic areas of visceral leishmaniasis in China in various culture media, so as to provide experimental evidence for selecting an appropriate medium for the culture of .

Methods: A total of 3 × 105 promastigotes of KS-2, Cy and JIASHI-5 isolates were inoculated into 1 mL NNN medium, 1 mL M199 medium supplemented with 20% fetal bovine serum medium, 1 mL M199 medium supplemented with 20% horse serum medium, and 1 mL brain heart infusion medium containing heme, respectively. All media were placed at 22 ℃ under a sterile condition, and the number of promastigotes was counted continuously for 8 days under a microscope.

[Comparative proteomics analysis of strains isolated from two epidemiological regions of visceral leishmaniasis in China].

Objective: To analyze the protein abundance differences between two strains isolated from different epidemiological types of visceral leishmaniasis in China by comparative proteomics method.

Methods: Tryptic digests of total proteins were analyzed by using liquid chromatography-tandem mass spectrometry (LC-MS/MS), followed by label-free quantitative differential expression analysis. The MS data were analyzed with MaxQuant software (ver 1.

Field evaluation of an immunochromatographic test for diagnosis of cystic and alveolar echinococcosis.

Background: The larval stages of the tapeworms Echinocoocus granulosus and Echinococcus multilocularis are the causative agents of human cystic echinococcosis (CE) and human alveolar echinococcosis (AE), respectively. Both CE and AE are chronic diseases characterised by long asymptomatic periods of many years. However, early diagnosis of the disease is important if treatment and management of echinococcosis patients are to be successful.

[Interpretation of Diagnostic Criteria for Kala-azar].

Kala-azar was once transmitted in the northern area of the Yangtze River in China, including 16 provinces (cities or autonomous regions). Through the great continuing prevention and control effort, this disease has been effectively controlled in the most of endemic areas. However, because the epidemic factors of the disease are complex, this disease still transmits or sporadically occurs in the western part of China, including 60 counties of Xinjiang, Gansu, Sichuan, Inner Mongolia, Shanxi, and Shaanxi provinces (autonomous regions).

[Cloning, expression and serological evaluation of H3 protein from ].

Objective: To clone and express the basement membrane specific heparan sulfate proteoglycan core protein (H3), and to evaluate its effect in detection of human cystic echinococcosis (CE).

Methods: The H3 gene immunoscreened from the cDNA library was cloned into pGEX-4T vector. The recombinant plasmid pGEX-3X-AgB8/3 was transformed into BL21 strains and induced by isopropyl-β-D-thiogalactopyranoside (IPTG).

[Analysis of Antibodies with Superior Antigen-recognition in Serum from Patients Infected by Necator americanus].

Objective: To evaluate the reactivity of adult hookworm antigens to serum from patients with hookworm disease, and analyze in the serum class- or subclass-specific antibodies that show superior antigen recognition.

Methods: Sera from healthy participants, patients infected by Necator americanus and those with other parasitic infections were processed for ELISA, which used raw antigens extracted from adult worms of Necator americanus as the coating antigen, and different classes or subclasses of anti-human antibody labeled with HRP as the secondary antibody. The sensitivity and specificity of the assay with various secondary antibodies were compared.

Visceral Leishmaniasis in China: an Endemic Disease under Control.

Visceral leishmaniasis (VL) caused by Leishmania spp. is an important vector-borne and largely zoonotic disease. In China, three epidemiological types of VL have been described: anthroponotic VL (AVL), mountain-type zoonotic VL (MT-ZVL), and desert-type ZVL (DT-ZVL).

[Molecular networks and mechanisms of epithelial-mesenchymal transition regulated by miRNAs in the malignant melanoma cell line].

Melanoma is a malignant cutaneous cancer of high metastasis and lethal rates. Epithelial-mesenchymal transition (EMT) plays an important role in the embryonic developmental process that is often activated during tumorigenesis and metastasis. In this study, we integrated of mRNA and miRNA transcriptome sequencing data of melanocyte and melanoma cell lines to identify genes involved in the process of tumor EMT in the first place, and uncovered 11 miRNAs including miR-130a-3p, miR-130b-3p, miR-125a-5p, miR-30a-3p, miR-195-5p, miR-345-5p, miR-509-3-5p, miR-374a-5p, miR-509-5p, miR-148a-3p and miR-330-3p, negatively related with EMT genes using the Mirsystem software.

Survey of Wild and Domestic Mammals for Infection with Leishmania infantum following an Outbreak of Desert Zoonotic Visceral Leishmaniasis in Jiashi, People's Republic of China.

In 2008 and 2009, an outbreak of desert-subtype zoonotic visceral leishmaniasis occurred in Jiashi county, Xinjiang, China. So far, no animal reservoir has been identified for this type of visceral leishmaniasis. Therefore, we surveyed the most common mammals (wild and domestic) for Leishmania infections during the outbreak in 2008 and 2009 in order to identify the source of the visceral leishmaniasis in this region.

Development of a LAMP assay for detection of Leishmania infantum infection in dogs using conjunctival swab samples.

Background: Leishmania infantum infections in dogs play a crucial role in the transmission of pathogens causing visceral leishmaniasis to humans in the Gansu province, northwest China. To be able to control zoonotic transmission of the parasite to humans, a non-invasive loop-mediated isothermal amplification (LAMP) assay to specifically detect L. infantum infections in dogs was developed.

Development of an Immunochromatographic Test for Diagnosis of Visceral Leishmaniasis Based on Detection of a Circulating Antigen.

Background: Visceral leishmaniasis (VL) is a life-threatening disease caused by protozoan parasites of the Leishmania donovani complex. Early case detection followed by adequate treatment is essential to the control of VL. However, the available diagnostic tests are either invasive and require considerable expertise (parasitological demonstration of the parasite in tissue smears) or unable to distinguish between past and active infection (serological methods).

Differential diagnosis of cystic and alveolar echinococcosis using an immunochromatographic test based on the detection of specific antibodies.

Human cystic and alveolar echinococcoses are zoonotic diseases caused by the larval stages of Echinococcus granulosus and Echinococcus multilocularis, respectively. As the diseases are co-endemic in many areas of the world, a simple and rapid test for the differential diagnosis of cystic echinococcosis (CE) and alveolar echinocoocosis (AE) is needed. Here, we describe the development of an immunochromatographic test (ICT) using crude hydatid cyst fluid and a recombinant 18-kDa protein (rEm18) as antigens for the detection of E.

[Research progress on the diagnosis technology of canine visceral leishmaniasis].

Canine visceral leishmaniasis is caused by Leishmania infantum. Infected dogs, either symptomatic or asymptomatic, are considered as the major reservoirs for zoonotic visceral leishmaniasis. Accurate and rapid detection of canine leishmanial infection is crucial for control of human visceral leishmaniasis due to its role in the transmission of the infection to vectors.

[Rapid risk assessment on the import of American trypanosomiasis to China].

American trypanosomiasis, as one of the "neglected tropical diseases", is a zoonosis induced by Trypanosoma cruzi. It is endemic in 18 countries in the Central and South America, especially in rural areas. A rapid risk assessment was carried out to analyze the potential threat of imported cases to China, which would provide information to policy makers in health authorities.

[Cloning, expression and bioinformatics analysis of cyclophilin of Echinococcus granulosus].

Objective: To clone and express EgCyP gene of Echinococcus granulosas and analyze EgCyP using bioinformatics.

Methods: Total RNAS of adult E. granulosus was extracted and reversedly transcripted to cDNA.

[Establishment and evaluation of colloid gold labeled immunochromatographic strip test for rapid diagnosis of alveolar echinococcosis].

Objective: To establish and evaluate a colloid gold immunochromatographic strip test for the diagnosis of alveolar echinococcosis.

Methods: Total RNA was prepared from Echinococcus multilocularis protoscoleces collected from Xinjiang Uygur Autonomous Region. Em18 gene was obtained by reverse transcription-polymerase chain reaction (RT-PCR).

Current epidemiological profile and features of visceral leishmaniasis in people's republic of China.

Background: Visceral leishmaniasis (VL) is still an important public health problem in China. In recent years endemic regions spread, prevalence increased, and even an outbreak of the disease occurred in China due to global warming and population movement. It is essential to elucidate the current epidemic situation and epidemiological characteristics of VL for designing control policy.

[Detection and species identification of two imported cases of cutaneous leishmaniasis].

Objective: To diagnose and identify pathogen of two suspected cases of cutaneous leishmaniasis.

Methods: Two cases of dermatosis with several major ulcers on the skin were examined, who worked and returned from Algeria (case 1) and Saudi Arabia (case 2), respectively. The stained smears of skin tissue from lesions were observed by microscope.

The prevalence of canine Leishmania infantum infection in western China detected by PCR and serological tests.

Background: Canine leishmaniasis (CanL) is endemic in western China, resulting in important public health problem. It is essential to evaluate the prevalence of canine Leishmania infantum infection for designing control policy. In the present study we report for the first time prevalence of Leishmania infection in dogs living in Jiuzhaigou County (Sichuan Provence, China), which is not only an important endemic area of CanL but also a tourism scenic spot, detected by PCR, ELISA and dipstick test.

An outbreak of the desert sub-type of zoonotic visceral leishmaniasis in Jiashi, Xinjiang Uygur Autonomous Region, People's Republic of China.

Few outbreaks of the desert sub-type of zoonotic visceral leishmaniasis (VL) have been described worldwide. In 2008, the incidence rate of VL in Jiashi County, Xinjiang Uygur Autonomous Region in the western part of the People's Republic of China, increased more than twenty-folds compared to the average annual incidence rate. The majority of the cases (96.

[Field evaluation of gold-immunochromatographic assay for diagnosis of vivax malaria].

Objective: To evaluate a gold-immunochromatographic assay (GICA) for malaria diagnosis in an endemic area of vivax malaria.

Methods: Blood samples were collected from febrile patients in 5 township-hospitals of Mengcheng County, Anhui Province, between September and October 2008. The samples were examined by GICA and microscopy under double blind condition and the results were compared.