Bioinertization of NanoLC/MS/MS Systems by Depleting Metal Ions From the Mobile Phases for Phosphoproteomics.

We have successfully developed a bioinertized nanoflow LC/MS/MS (nanoLC/MS/MS) system for the highly sensitive analysis of phosphopeptides by depleting metal ions from the mobile phase. We found that not only direct contact of phosphopeptides with metal components, but also indirect contact with nanoLC pumps through the mobile phase causes significant losses during the recovery of phosphopeptides. Moreover, electrospray ionization was adversely affected by the mobile phase containing multiple metal ions as well as by the sample solvents contaminated with metal ions used in immobilized metal ion affinity chromatography for phosphopeptide enrichment.

Z(eff) measurement using extreme ultraviolet bremsstrahlung emission in LHD.

Radial profile measurement of Z(eff) using visible bremsstrahlung (5300 Å) in the Large Helical Device (LHD) has often encountered difficulties because the intensity profile was largely deformed by the nonuniform visible bremsstrahlung emissions from the edge ergodic layer surrounding the core plasma. A space-resolved flat-field extreme ultraviolet (EUV) spectrometer has been newly adopted to measure the Z(eff) profile using the EUV bremsstrahlung continuum in the wavelength range of 70-75 Å. The EUV bremsstrahlung intensity profiles have been measured and checked for all the magnetic configurations with totally different magnetic field structures in the ergodic layer of LHD.