Publications by authors named "Julio Pozo"
Article Synopsis
- Systemic mastocytosis (SM) is a complex disease linked to abnormal mast cells that release mediators affecting the immune environment.
- The study aimed to explore the blood profiles of B-cells, plasma cells, and antibody types in 108 SM patients compared to healthy individuals.
- Results showed increased immature B-cells and elevated IgM and IgD levels in SM patients, alongside decreased plasma cells across various IgH types, with unique immune patterns based on the SM subtype.
Purpose: Natural killer (NK) cells are traditionally identified by flow cytometry using a combination of markers (CD16/CD56/CD3), because a specific NK-cell marker is still missing. Here we investigated the utility of CD314, CD335 and NKp80, compared to CD16/CD56/CD3, for more robust identification of NK-cells in human blood, for diagnostic purposes.
Methods: A total of 156 peripheral blood (PB) samples collected from healthy donors (HD) and patients with diseases frequently associated with loss/downregulation of classical NK-cell markers were immunophenotyped following EuroFlow protocols, aimed at comparing the staining profile of total blood NK-cells for CD314, CD335 and NKp80, and the performance of distinct marker combinations for their accurate identification.
Post-pregnancy breast cancer often carries a poor prognosis, posing a major clinical challenge. The increasing trend of later-life pregnancies exacerbates this risk, highlighting the need for effective chemoprevention strategies. Current options, limited to selective estrogen receptor modulators, aromatase inhibitors, or surgical procedures, offer limited efficacy and considerable side effects.
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- Systemic mastocytosis (SM) is a complex disease marked by an increase in abnormal mast cells that can affect the immune system and tumor environment.
- Researchers analyzed blood samples from 115 SM patients and 83 healthy individuals to assess the distribution of various immune cells and their relationship with disease characteristics.
- Findings indicated that SM patients had reduced levels of specific immune cell types compared to healthy controls, with these variations depending on the subtype of SM and certain genetic factors linked to the disease.
Monoclonal gammopathy of undetermined significance (MGUS) is the earliest discernible stage of multiple myeloma (MM) and Waldenström's macroglobulinemia (WM). Early diagnosis of MG may be compromised by the low-level infiltration, undetectable to low-sensitive methodologies. Here, we investigated the prevalence and immunophenotypic profile of clonal (c) plasma cells (PC) and/or cB-lymphocytes in bone marrow (BM) and blood of subjects with a serum M-component from the iSTOPMM program, using high-sensitive next-generation flow cytometry (NGF), and its utility in the diagnostic classification of early-stage MG.
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- Low-count monoclonal B-cell lymphocytosis (MBL) is linked to a higher risk of severe COVID-19 and potential immunodeficiency.
- A study involving 336 COVID-19 patients showed that MBL patients had increased counts of certain immune cells, especially plasma cells, but decreased counts of others like lymphocytes, particularly in the early stages of infection.
- MBL patients also exhibited a delayed but stronger antibody response compared to non-MBL patients, indicating distinct immune responses in the context of COVID-19.