Inactivation of Tor proteins affects the dynamics of endocytic proteins in early stage of endocytosis.

Tor2 is an activator of the Rom2/Rho1 pathway that regulates alpha-factor internalization. Since the recruitment of endocytic proteins such as actin-binding proteins and the amphiphysins precedes the internalization of alpha-factor, we hypothesized that loss of Tor function leads to an alteration in the dynamics of the endocytic proteins. We report here that endocytic proteins, Abp1 and Rvs167, are less recruited to endocytic sites not only in tor2 but also tor1 mutants.