A methodological proposal to estimate the total abundance of immature mosquitoes in discarded tyres: Aedes aegypti and Culex pipiens as study cases.

Estimating the abundance of immature mosquitoes in discarded water-filled tyres is a laborious task due to their shape and size. Our objective was to develop a procedure that allows, by counting individuals in a water sample, to estimate their total abundance. Polynomial functions linking water column height and water volume were fitted for five tyre categories (from cars to tractors) and horizontal/vertical storage positions.