The arrangement of dual-species biofilms of and can be modified by the medium: effect of Voriconazole.

Both and have been isolated from different types of infections over the years. They have the ability to form communities of microorganisms known as biofilms. It has been demonstrated that the medium employed in studies may affect the biofilm development.

The inhibitory effect of photodynamic therapy on dual-species biofilms of Candida albicans and Candida krusei can be determined by Candida albicans/Candida krusei ratio.

Candida krusei and Candida albicans present the ability to form communities of microorganisms called biofilms. Biofilms can be composed of a single species or more and are an important virulence factor. The inhibition of C.

Effect of antimicrobial photodynamic therapy using toluidine blue on dual-species biofilms of Candida albicans and Candida krusei.

Background: Although Candida albicans is the most frequent etiological agent of candidiasis, it has been reported a sizable number of infections related to the non-albicans Candida (NAC) species, Candida krusei. In addition, dual biofilms (biofilms composed by two species) may easily occur in vivo, becoming even more challenging the treatment of an infection. The fungicide effect of Photodynamic Therapy (PDT), using toluidine blue O (TBO) on both C.

(MeOPhSe)2, a synthetic organic selenium compound, inhibits virulence factors of Candida krusei: Adherence to cervical epithelial cells and biofilm formation.

Background: Systemic candidiasis is produced by Candida albicans or non-albicans Candida species, opportunistic fungi that produce both superficial and invasive infections. Despite the availability of a wide range of antifungal agents for the treatment of candidiasis, failure of therapy is observed frequently, which opens new avenues in the field of alternative therapeutic strategies.

Methods: The effects of p,p'-methoxyl-diphenyl diselenide [(MeOPhSe)], a synthetic organic selenium (organochalcogen) compound, were investigated on virulence factors of C.

Diphenyl diselenide suppresses key virulence factors of , a neglected fungal pathogen.

is a candidiasis etiological agent of relevance in the clinical setting because of its intrinsic resistance to fluconazole. Also, it has opened up new paths in the area of alternative therapeutic techniques. This project demonstrated the effects of diphenyl diselenide (PhSe) and -cloro diphenyl diselenide (Cl-PhSe), two organochalcogen compounds, on relevant virulence factors for the early stage of the host interaction and infection process.

(PhSe) and (Cl-PhSe) organochalcogen compounds inhibit adhesion to human endocervical (HeLa) cells and show anti-biofilm activities.

Adhesion capacity on biological surfaces and biofilm formation is considered an important step in the infection process by The ability of (PhSe) and (Cl-PhSe), two synthetic organic selenium (organochalcogen) compounds, to act on virulence factors related to adhesion to human endocervical (HeLa) cell surfaces and their anti-biofilm activities was analyzed. Both organochalcogen compounds inhibited adhesion to HeLa cells, dependent on compound concentrations. (PhSe) (at 20 µM;  = 0.

BSA nanoparticles loaded-methylene blue for photodynamic antimicrobial chemotherapy (PACT): effect on both growth and biofilm formation by .

It has been demonstrated an increase in resistance of to conventional therapies, probably, due the indiscriminate use of the conventional antifungal drugs. In this aspect, the nanotechnology generates the possibility of creating new therapeutic agents. Thus, the objective of this paper was to produce and characterize a bovine serum albumin (BSA) nanoparticle encapsulated with Methylene Blue (MB).

Photodynamic Antimicrobial Chemotherapy (PACT), using Toluidine blue (TBO) inhibits both growth and dimorphism in Paracoccidioides brasiliensis by a mechanism involving reactive oxygen species (ROS) production.

Background: The thermo-dimorphic fungus Paracoccidioides brasiliensis is the pathogen of Paracoccidioidomycosis, an important public health problem in Latin American with prevalence in Brazil. Photodynamic Antimicrobial Chemotherapy (PACT) is a process that combines a photosensitizer and light, producing reactive oxygen species (ROS) that can promote damages to treated cells.

Methods: In this work was study the effect of PACT, using Toluidine blue (TBO) on both yeast and mycelial cells of P.