Publications by authors named "Julien Bessonnat"
Article Synopsis
- Numerical chromosomal aberrations in sperm are a leading cause of infertility and developmental issues in mammals, including primates; however, their prevalence in non-human primates like chimpanzees is not well studied.
- This research investigates sperm aneuploidy in chimpanzees using custom probes and sperm-FISH analysis on over 10,000 sperm nuclei, revealing specific rates of disomy and gamete proportions.
- The findings represent the first comprehensive overview of sperm aneuploidy in non-human primates, enhancing understanding of its origins and mechanisms.
Article Synopsis
- Human ovarian tissue cryopreservation (OTC) is a method used to preserve female fertility for prepubertal girls and women facing treatments that could lead to premature ovarian insufficiency (POI), but there's a lack of standard protocols.
- * The manuscript presents a protocol that not only involves freezing ovarian tissue but also the cryopreservation of both mature and immature oocytes, enhancing the chances of successful fertility preservation.
- * It emphasizes the importance of proper handling and storage during the process and recommends this combined approach to maximize reproductive potential and improve safety outcomes for patients at risk of POI.
Article Synopsis
- Globozoospermia is a type of male infertility characterized by round-headed sperm that lack an acrosome, with genetic anomalies in the DPY19L2 gene accounting for 50-70% of cases, often due to complete gene deletions.* -
- A study involving 69 patients found that 36% had homozygous deletions of the DPY19L2 gene, and even higher proportions of genetic defects correlated with increased rates of globozoospermia, indicating a strong relationship between genotype and phenotype.* -
- The study suggests a new diagnostic approach focusing on patients with over 50% globozoospermia by initially testing for DPY19L2 deletions using
Article Synopsis
- - PROK1 levels in individual follicular fluid (FF) have been identified as a promising biomarker to predict the competence of oocytes in IVF, with a strong correlation to follicular size.
- - A study conducted at the University Hospital of Grenoble with 69 infertile couples confirmed that higher PROK1 concentrations in FF are indicative of better embryo development potential, independent of other embryo growth indicators.
- - The research suggests that hormones like Follicle Stimulating Hormone (FSH) and hCG can enhance PROK1 secretion, potentially affecting oocyte quality through biological pathways linked to cAMP.
Article Synopsis
- Robertsonian translocations (RobT) are common chromosome rearrangements where carriers often produce mostly balanced sperm through a process called alternate segregation, raising questions about the relevance of sperm FISH analysis for these individuals.* -
- A study analyzed 23 patients from 2003-2017 alongside 187 cases from literature, finding that 73.45% of spermatozoa from RobT carriers were chromosomally balanced, in line with existing data, although some translocation types, particularly der(13;15), showed lower rates.* -
- Results suggest that despite some variations in sperm quality among RobT carriers, sperm FISH analysis remains important for genetic counseling and assessing reproductive options for these patients.*
Spermaurin, an La1-like peptide from the venom of the scorpion Scorpio maurus palmatus, improves sperm motility and fertilization in different mammalian species.
Guillaume Martinez Jean-Pascal Hograindleur Sébastien Voisin Roland Abi Nahed Tarek M Abd El Aziz Julien Bessonnat
Mol Hum Reprod
February 2017
Article Synopsis
- Researchers explored the venom of the scorpion Scorpio maurus palmatus to find compounds that could improve sperm motility, which is crucial for fertilization success.
- A specific peptide containing disulfide bonds (73 amino acids long) was isolated, showing significant enhancement of sperm motility in multiple mammalian species, including humans.
- The study involved analyzing various venom fractions, purifying the effective peptide, and testing its impact on sperm from both human and animal sources, revealing its potential applications in treating fertility issues.
Article Synopsis
- Prokineticin 1 (PROK1) is important for endometrial receptivity and placental development, yet its potential as a noninvasive biomarker for embryo implantation is still unproven.
- The study aimed to connect PROK1 levels in follicular fluid and fertilization culture media with reproductive outcomes in IVF patients, as well as examine the expression of PROK1 and its receptors in the follicles.
- Results indicated that higher PROK1 levels were associated with successful embryo implantation, showing predictive value, while the expression of the PROK1/PROKR system was confirmed during folliculogenesis.
Article Synopsis
- - The study examined the practice of sperm freezing in patients with very low sperm counts (cryptozoospermia/severe oligozoospermia) undergoing Intracytoplasmic Sperm Injection (ICSI) over an 8-year period, aiming to assess the usage of frozen sperm. - Out of 169 patients, the majority (84) chose to freeze their sperm before ICSI while 85 did not, with an increase in sperm freezing practices observed from 29% to 74% between 2004 and 2011, yet only 5.9% of cases involved the use of frozen sperm. - The median age of patients who did not freeze their sperm was significantly higher, but there was