Publications by authors named "Julie Saule"
Objectives: We evaluated a real-time quantitative PCR (qPCR) for detection of the (TP) genome in clinical samples through simultaneous detection of two genomic targets.
Methods: We performed qPCR with TaqMan technology using two TP genes, and , as targets, with an internal positive control. The qPCR assay was compared with syphilis diagnosis based on a combination of clinical examination, serological results and inhouse nested PCR (nPCR).
Article Synopsis
- Benzathine penicillin G (BPG) is the standard treatment for early syphilis, but recent shortages have prompted the need for alternative therapies.
- A study in France assessed genetic resistance of the syphilis-causing bacteria, Treponema pallidum, to macrolides and doxycycline using swabs from 146 patients.
- Results showed a high level of macrolide resistance (85% of samples), while no resistance to doxycycline was found, suggesting doxycycline is a viable substitute for BPG in treating early syphilis.