Survival of Sclerotia in Central New York.

White mold caused by is a serious disease affecting many field and specialty crops in New York (NY). The primary inoculum for white mold is sclerotia, which are hardened masses of mycelia that survive adverse environmental conditions and periods of nonhosts. However, NY crop guidelines lack rotation and residue management recommendations based on local knowledge of sclerotial survival.

, a New iOS Application for Assessment of Damage by Diseases and Insect Pests Using Sequential Sampling Plans.

Regular scouting for plant diseases and insect pests by growers, crop consultants, extension educators, and researchers (herein defined as stakeholders) is the cornerstone of integrated pest management practices. Sequential sampling plans have the potential to save time and labor in field scouting and reduce the frequency of errors surrounding decision-making. The incorporation of the algorithms behind sequential sampling plans into mobile devices can make scouting for diseases and insect pests more straightforward, practical, and enjoyable.

Control of Phoma Leaf Spot and Root Decay of Table Beet in New York.

Disease caused by (syn. , ) results in reductions in plant populations, foliar disease (Phoma leaf spot [PLS]), and root disease and decay in table beet. Disease caused by has reemerged as prevalent in organic table beet production in New York.

Development of a Sequential Sampling Plan using Spatial Attributes of Cercospora Leaf Spot Epidemics of Table Beet in New York.

Sampling strategies that effectively assess disease intensity in the field are important to underpin management decisions. To develop a sequential sampling plan for the incidence of Cercospora leaf spot (CLS), caused by , 31 table beet fields were assessed in the state of New York. Assessments of CLS incidence were performed in six leaves arbitrarily selected in 51 sampling locations along each of three to six linear transects per field.

Optimizing Cercospora Leaf Spot Control in Table Beet Using Action Thresholds and Disease Forecasting.

Cercospora leaf spot (CLS), caused by the fungus is the dominant foliar disease affecting table-beet production in New York. CLS epidemics occur annually and, if uncontrolled, will rapidly lead to defoliation. In broad-acre production, season-long maintenance of healthy leaves is important to facilitate harvest by top-pulling.

Alternative Hosts of in Field Surveys and Inoculation Trials.

, the cause of Cercospora leaf spot (CLS) of sugar beet and table beet, has a broad range of potential alternative hosts. The role of these hosts as inoculum sources in the field is unclear and has had limited investigation since the advent of DNA-based pathogen identification. The presence of on alternative hosts associated with table beet fields of New York was assessed in field surveys during 2016.

Genetic Diversity and Structure in Regional Populations from subsp. Suggest Two Clusters of Separate Origin.

Cercospora leaf spot, caused by , is a highly destructive disease of subsp. worldwide. populations are usually characterized by high genetic diversity, but little is known of the relationships among populations from different production regions around the world.

Spatiotemporal Attributes and Crop Loss Associated with Tan Spot Epidemics in Baby Lima Bean in New York.

Tan spot, caused by the pycnidial fungi Didymella americana and Boeremia exigua var. exigua, is a foliar disease affecting processing baby lima bean production in New York. Tan spot epidemics are prevalent, occur annually, and may result in substantial defoliation.

Temporal Genetic Differentiation of Cercospora beticola Populations in New York Table Beet Fields.

Annual epidemics of Cercospora leaf spot (CLS), caused by the fungus Cercospora beticola, can result in substantial defoliation in table beet fields in New York. High allelic and genotypic diversity have been described within C. beticola populations; however, information on the temporal stability of populations is lacking.

IMA Genome-F 9: Draft genome sequence of (syn. ), two strains, , , cf and .

Draft genomes of the species (syn. , two strains, , , and are presented Both mating types (MAT1-1 and MAT1-2) of are included. Two strains of that produce sulfated homotyrosine echinocandin variants, FR209602, FR220897 and FR220899 are presented.

Cryptic diversity, pathogenicity, and evolutionary species boundaries in Cercospora populations associated with Cercospora leaf spot of Beta vulgaris.

The taxonomy and evolutionary species boundaries in a global collection of Cercospora isolates from Beta vulgaris was investigated based on sequences of six loci. Species boundaries were assessed using concatenated multi-locus phylogenies, Generalized Mixed Yule Coalescent (GMYC), Poisson Tree Processes (PTP), and Bayes factor delimitation (BFD) framework. Cercospora beticola was confirmed as the primary cause of Cercospora leaf spot (CLS) on B.

Global genotype flow in Cercospora beticola populations confirmed through genotyping-by-sequencing.

Genotyping-by-sequencing (GBS) was conducted on 333 Cercospora isolates collected from Beta vulgaris (sugar beet, table beet and swiss chard) in the USA and Europe. Cercospora beticola was confirmed as the species predominantly isolated from leaves with Cercospora leaf spot (CLS) symptoms. However, C.

Management of Cercospora Leaf Spot in Conventional and Organic Table Beet Production.

Cercospora leaf spot (CLS; Cercospora beticola) is the most important foliar disease affecting table beet. Epidemics occur annually and fungicides extend the survival of foliage to enable mechanized harvest. However, a high frequency of strobilurin-resistant C.

Genetic structure of Cercospora beticola populations on Beta vulgaris in New York and Hawaii.

Cercospora leaf spot (CLS), caused by Cercospora beticola, is a major disease of Beta vulgaris worldwide. No sexual stage is known for C. beticola but in its asexual form it overwinters on infected plant debris as pseudostromata, and travels short distances by rain splash-dispersed conidiospores.

Genotypic Diversity and Resistance to Azoxystrobin of Cercospora beticola on Processing Table Beet in New York.

Cercospora leaf spot (CLS), caused by Cercospora beticola, is one of the major diseases affecting productivity and profitability of beet production worldwide. Fungicides are critical for the control of this disease and one of the most commonly used products is the quinone outside inhibitor (QI) azoxystrobin. In total, 150 C.

Swede midge (Diptera: Cecidomyiidae), ten years of invasion of crucifer crops in North America.

The Swede midge, Contarinia nasturtii Kieffer (Diptera: Cecidomyiidae), a common insect pest in Europe, is a newly invasive pest in North America that constitutes a major threat to cruciferous vegetable and field crops. Since its first identification in Ontario, Canada, in 2000, it has rapidly spread to 65 counties in the provinces of Ontario and Quebec and has recently been found in canola (one of two cultivars of rapeseed, Brassica napus L. and Brassica campestris L.

Detection of Contarinia nasturtii (Diptera: Cecidomyiidae) in New York, a new pest of cruciferous plants in the United States.

The midge Contarinia nasturtii Kieffer (Diptera: Cecidomyiidae) was first confirmed in North America in Ontario, Canada, in 2000. The insect is now distributed throughout many counties in the provinces of Ontario and Québec. Nearly 1,200 farms in the northeastern United States that grow cruciferous vegetables are at risk for C.

Biolistic transformation of grapevine using minimal gene cassette technology.

The use of minimal gene cassettes (MCs), which are linear DNA fragments (promoter+open reading frame+terminator) lacking the vector backbone sequence, was compared to the traditional use of whole circular plasmids (CPs) for transformation of grapevine. Embryogenic cell suspensions of 'Chardonnay' (Vitis vinifera L.) were transformed via particle co-bombardment using two nonlinked genes in either MCs or CPs.

Evaluation of transgenic 'Chardonnay' (Vitis vinifera) containing magainin genes for resistance to crown gall and powdery mildew.

Magainins, short peptides with broad-spectrum antimicrobial activity in vitro, were assayed for their ability to confer resistance to pathogens in transgenic grapevines. Embryogenic cell suspensions of 'Chardonnay' (Vitis vinifera L.) were co-transformed by microprojectile bombardment with a plasmid carrying the npt-II gene and a second plasmid harboring either a natural magainin-2 (mag2) or a synthetic derivative (MSI99) gene.

In vitro germination and transient GFP expression of American chestnut (Castanea dentata) pollen.

The development of the male reproductive structures of American chestnut (Castanea dentata) is described to advance our understanding of its reproductive behavior. This information has been vital in the development of a strategy to collect pollen grains from male catkins suitable for in vitro germination and transformation experiments. Cutting male catkins into small segments and rolling them over a culture plate resulted in evenly dispersed and large amounts of pollen with minimal unwanted accessory floral parts.

Stable transformation of plant cells by particle bombardment/biolistics.

Particle bombardment, or biolistics, is a commonly used method for genetic transformation of plants and other organisms. Millions of DNA-coated metal particles are shot at target cells or tissues using a biolistic device or gene gun. The DNA elutes off the particles that lodge inside the cells, and a portion may be stably incorporated in the host chromosomes.