Publications by authors named "Julie Ming Liang"
Proteins comprise a multibillion-dollar industry in enzymes and therapeutics, but bacterial protein production can be costly and inefficient. Proteins of interest (POIs) must be extracted from lysed cells and inclusion bodies, purified, and resolubilized, which adds significant time and cost to the protein-manufacturing process. The Salmonella pathogenicity island 1 (SPI-1) type III secretion system (T3SS) has been engineered to address these problems by secreting soluble, active proteins directly into the culture media, reducing the number of purification steps.
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- * Experiments show that TFs Gcn4 and Nup2 directly interact with NPCs, while other factors linked to mRNA export or transcription play a more indirect role.
- * A special "positioning domain" in Gcn4 is crucial for its interaction with NPCs and can enhance the transcription of certain genes, indicating that a similar mechanism may apply to many yeast TFs.