Publications by authors named "Julie Meurisse"
The understanding that organelles are not floating in the cytosol, but rather held in an organized yet dynamic interplay through membrane contact sites, is altering the way we grasp cell biological phenomena. However, we still have not identified the entire repertoire of contact sites, their tethering molecules and functions. To systematically characterize contact sites and their tethering molecules here we employ a proximity detection method based on split fluorophores and discover four potential new yeast contact sites.
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- - The study examines how mitochondrial fusion is regulated through the interaction of the ubiquitin protease Ubp2 and ligases Mdm30 and Rsp5, focusing on their roles in maintaining mitochondrial integrity.
- - Ubp2 acts as an antagonist to Rsp5, which facilitates the production of fatty acid desaturase Ole1, while it also counteracts the degradation of the yeast mitofusin Fzo1 by Mdm30.
- - The findings suggest that the balance between Ubp2 and Mdm30 influences desaturated fatty acid levels, ultimately affecting Fzo1 stability and mitochondrial fusion, linking outer membrane fusion processes to lipid homeostasis.
Rad53 is a conserved protein kinase with a central role in DNA damage response and nucleotide metabolism. We observed that the expression of a dominant-lethal form of RAD53 leads to significant expression changes for at least 16 genes, including the RNR3 and the HUG1 genes, both of which are involved in the control of nucleotide metabolism. We established by multiple biophysical and biochemical approaches that Hug1 is an intrinsically disordered protein that directly binds to the small RNR subunit Rnr2.
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