The stability and elimination of mammalian enveloped and non-enveloped respiratory viruses in indoor air: Testing using a room-sized aerobiology chamber.

We assessed the viability of aerosolized human betacoronavirus OC43 (HCoV-OC43; ATCC VR-1558), human rhinovirus-14 (RV-14; ATCC VR-284) and feline calicivirus (FCV; ATCC VR-782) as representative enveloped and non-enveloped respiratory viruses of mammals in indoor air under ambient conditions (relative humidity 50±10% and air temperature 22±2°C) using a room-sized (25 m; 900 ft) aerobiology chamber. All virus suspensions contained a soil load to simulate the presence of body fluids and they were separately aerosolized into the chamber using a six-jet Collison nebulizer. A muffin fan was used to uniformly mix the air inside the chamber and to keep the aerosols airborne.