Fortifying fresh human milk with commercial powdered human milk fortifiers does not affect bacterial growth during 6 hours at room temperature.

Objective: To evaluate the growth of resident aerobic mesophilic flora and added Enterobacter sakazakii in fresh, unfortified human milk; fresh human milk fortified with two commercial powdered fortifiers differing in iron content; and infant formula prepared from powder.

Subjects: Eight mothers provided preterm breast milk samples.

Methods: Breast milk samples were divided into three aliquots: unfortified, fortified with fortifier containing 1.