Publications by authors named "Julie Huiyuan Xiao"
Article Synopsis
- - The study investigates the effectiveness of convalescent plasma (CP) therapy for COVID-19 by evaluating different immunoassays and their ability to predict neutralizing antibody levels in CP donations.
- - Researchers tested three immunoassays (EUROimmun, Ortho, Roche), a pseudotype-based assay, and two others assessing ACE2 binding to gauge their efficacy in determining antibody strength across 113 CP samples.
- - All assays effectively identified high-titer donations, but their ability to predict neutralizing antibody levels varied, with the pseudotype assay showing the best correlation (0.61), suggesting they could be used for selecting CP donations.
Use of dual sgRNAs is a common CRISPR/Cas9-based strategy for the creation of genetic deletions. The ease of screening combined with a rather high rate of success makes this approach a reliable genome engineering procedure. Recently, a number of studies using CRISPR/Cas9 have revealed unwanted large-scale rearrangements, duplications, inversions or larger-than-expected deletions.
View Article and Find Full Text PDFWe have produced a new Ebola virus pseudotype, E-S-FLU, that can be handled in biosafety level 1/2 containment for laboratory analysis. The E-S-FLU virus is a single-cycle influenza virus coated with Ebolavirus glycoprotein, and it encodes enhanced green fluorescence protein as a reporter that replaces the influenza virus hemagglutinin. MDCK-SIAT1 cells were transduced to express Ebolavirus glycoprotein as a stable transmembrane protein for E-S-FLU virus production.
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