Estimation of the accuracy of an ELISA test applied to bulk tank milk for predicting herd-level status for Salmonella Dublin in dairy herds using Bayesian Latent Class Models.

Enzyme-Linked Immunosorbent Assay (ELISA) test is commonly used for detection of antibodies to Salmonella Dublin in individual bovine milk samples. However, little is known about its accuracy when used on bulk tank milk for determining herd-level S. Dublin status and when evaluated without assuming a perfect reference test.

Repeatability of a Commercially Available ELISA Test for Determining the Herd-Level subsp. Serovar Dublin Status in Dairy Herds Using Bulk Milk.

An Enzyme-Linked Immunosorbent Assay (ELISA) is currently available for detection of antibodies against Dublin in bovine milk. However, when used in a surveillance program, samples may undergo various storage conditions. The objective of this study was to estimate the repeatability of an ELISA test when used on fresh and frozen samples.