Determination of neurosteroids in human cerebrospinal fluid in the 21st century: A review.

Determination of steroid hormones synthesized by the human body plays an important role in various fields of endocrinology. Neurosteroids (NS) are steroids that are synthesized in the central (CNS) or peripheral nervous system (PNS), which is not only a source but also a target for neurosteroids. They are discussed as possible biomarkers in various cognitive disorders and research interest in this topic raises continuously.

Splitless hyphenation of SFC with MS by APCI, APPI, and ESI exemplified by steroids as model compounds.

A systematic evaluation of splitless hyphenation of supercritical fluid chromatography (SFC) with mass spectrometry (MS) was performed using different techniques for ambient pressure ionization. Interfaces commonly known from HPLC-MS/MS, i.e.

Methods in endogenous steroid profiling - A comparison of gas chromatography mass spectrometry (GC-MS) with supercritical fluid chromatography tandem mass spectrometry (SFC-MS/MS).

In various fields of endocrinology, the determination of steroid hormones synthesised by the human body plays an important role. Research on central neurosteroids has been intensified within the last years, as they are discussed as biomarkers for various cognitive disorders. Their concentrations in cerebrospinal fluid (CSF) are considered to be regulated independently from peripheral fluids.