Effect of different application frequencies of CPP-ACP and fluoride dentifrice on demineralized enamel: a laboratory study.

Purpose: To evaluate the effect of different application frequencies of dentifrices containing CPP-ACP and fluoride on enamel demineralization inhibition using a pH cycling model.

Methods: A total of 120 enamel blocks obtained from permanent human third molars were divided into eight groups according to the treatment and number of times that the dentifrice slurry was applied (three or five times): Control--Dentifrice without Fluoride (CO3 and CO5); Fluoride Dentifrice (FD3 and FD5--commercial dentifrice 1100 ppm as NaF); MI Paste (MP3 and MP5--Recaldent) and MI Paste Plus (MPP3 and MPP5--Recaldent 900 ppm as NaF). The specimens were alternately soaked in a demineralizing solution for 6 hours and in a remineralizing solution for 18 hours for 5 days.