Chagas Immunochromatographic Rapid Test in the Serological Diagnosis of Infection in Wild and Domestic Canids.

(domestic dog) represents a reliable sentinel for the occurrence of a well-established transmission cycle of among wild mammals in the surroundings and, consequently, where the risk of human infection exists. Serological diagnosis is the chosen method to identify infection in dogs that, in Brazil, rarely present positive parasitological tests. The use of recombinant chimeric parasitic antigens results in a sensitive and specific serological diagnostic test in contrast to the use of crude antigens.

sp. n.: A species of the clade associated with the neotropical common vampire bat .

Molecular phylogenetic studies have revealed the growing diversity of bat trypanosomes. Here, 14 isolates from blood samples of the vampire bat (Phyllostomidae) from Rio de Janeiro, Southeast Brazil, were cultivated, and morphologically and molecularly characterized. All isolates represent a novel species named n.

Biological study of Trypanosoma caninum under co-culture with different feeder layer cells.

Trypanosoma caninum is a parasite isolated from domestic dogs, of which several biological aspects remain unknown, including evolutive forms found in vertebrate hosts. The objective of this study was to evaluate co-cultures of T. caninum with different cell lines as feeder layers to monitor the differentiation process and investigate infective potential.

sp. nov. (Protozoa: Kinetoplastida) isolated from (Acari: Ixodidae) ticks in Rio de Janeiro, Brazil.

Parasites of the genus are microorganisms that display wide morphological, biological and genetic variability. Here we present the first description of an isolate of the genus naturally infecting the tick . The ticks were collected from a specimen of (Queixada, white-lipped peccary) from the Itatiaia National Park, Itatiaia, Rio de Janeiro, Brazil.

Report of new cases of Trypanosoma caninum in Brazil.

Introduction: Trypanosoma caninum is a protozoan species recently described in dogs, whose occurrence has been reported in areas of overlap with visceral leishmaniasis.

Methods: Trypanosoma sp. were isolated from nine dogs and characterized by molecular methods.

Molecular study of Trypanosoma caninum isolates based on different genetic markers.

Trypanosoma caninum is a parasite recently described in dogs, whose life cycle is rather unknown. Here, we performed a genetic study with T. caninum samples obtained in different Brazilian regions.

Aflagellar epimastigote forms are found in axenic culture of Trypanosoma caninum.

Representatives of the genus Trypanosoma have been traditionally found in epimastigote, espheromastigote and trypomastigote flagellated forms in axenic cultures. Trypanosoma caninum is a trypanosomatid that has recently been reported infecting dogs in endemic areas of canine leishmaniasis in Brazil. It presents specific biological characteristics and it is found exclusively on healthy skin.

Trypanosoma caninum, a new parasite described in dogs in Brazil: aspects of natural infection.

Trypanosoma caninum constitutes the most recent trypanosomatid species infecting dogs in Brazil. Due to the limited data available about this parasite, this study aims to disclose clinical and laboratory findings from 14 dogs naturally infected. The dogs were diagnosed during a cross-sectional survey in Cuiabá (Mato Grosso, Brazil) and followed up at an interval of 3, 6, and 12 mo in order to evaluate the clinical evolution and to investigate the parasite, the DNA, or both in different biological samples (intact skin, cutaneous scar, blood, bone marrow, and lymph node aspirate) by parasitological (culture and smear exam) and molecular (DNA-based tests) methods.