Immunolocalization for glucocorticoid receptor and effect of cortisol on development of preantral follicles.

The aim of this study was to evaluate the immunolocalization for glucocorticoid receptor (NR3C1) in goat ovarian follicles and the effect of cortisol on development of preantral follicles. Goat ovarian fragments were cultured for 7 days under different cortisol concentrations (0, 1, 5 and 10 ng/ml). Before and after culture, the protein expression of NR3C1 was analyzed in ovarian tissue by immunohistochemical analysis.