Dual antibacterial drug-loaded nanoparticles synergistically improve treatment of Streptococcus mutans biofilms.

Dental caries (i.e., tooth decay), which is caused by biofilm formation on tooth surfaces, is the most prevalent oral disease worldwide.

Rigor and reproducibility in polymer nanoparticle synthesis and characterization.

Standardized process improvement methods and tools were used to enhance the rigor and reproducibility of diblock copolymer nanoparticle (NP) synthesis and characterization. Models linking design parameters with NP characteristics boosted process control for NP synthesis, which may improve translation and commercialization of NP research.

Evaluation of Recombinant Bone Morphogenetic Protein-2 Bioactivity for Regenerative Medicine.

This work is a systematic evaluation of the experimental parameters of the most widely used recombinant human bone morphogenetic protein-2 (rhBMP-2) activity assays. The variations in assays reported in the literature have challenged the reproducibility and translation of work using rhBMP-2 as a bone-inducing growth factor. By elucidating the effect of model cell line on the dose-dependent alkaline phosphatase response to rhBMP-2 induction and by establishing a correlation between protein activity and protein concentration by enzyme-linked immunosorbent assay using commercially available rhBMP-2, this work is a significant step toward developing an correlation between quantified activity and clinical efficacy.