Publications by authors named "Julian C Krappinger"
The methylotrophic yeast Komagataella phaffii is a popular host system for the pharmaceutical and biotechnological production of recombinant proteins. CRISPR-Cas9 and its derivative CRISPR interference (CRISPRi) offer a promising avenue to further enhance and exploit the full capabilities of this host. MAD7 and its catalytically inactive variant "dead" MAD7 (dMAD7) represent an interesting alternative to established CRISPR-Cas9 systems and are free to use for industrial and academic research.
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- Placenta-specific trophoblast cells and tumor cells share characteristics like tissue invasion and immune system evasion, which are crucial for their survival and function.
- Both cell types are influenced by their surrounding microenvironments, impacting their ability to invade, form new blood vessels (angiogenesis), and avoid detection by the immune system.
- Unlike tumor cells, trophoblast cells have more stringent regulatory mechanisms governing their metabolism, proliferation, migration, and invasiveness, leading to distinct differences in their cellular behavior.
Non-coding natural antisense transcripts (ncNATs) are regulatory RNA molecules that are overlapping with as well as complementary to other transcripts. These transcripts are implicated in a broad variety of biological and pathological processes, including tumorigenesis and oncogenic progression. With this complex field still in its infancy, annotations, expression profiling and functional characterisations of ncNATs are far less comprehensive than those for protein-coding genes, pointing out substantial gaps in the analysis and characterisation of these regulatory transcripts.
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