Comparison of relative mRNA quantification models and the impact of RNA integrity in quantitative real-time RT-PCR.

Relative quantification in quantitative real-time RT-PCR is increasingly used to quantify gene expression changes. In general, two different relative mRNA quantification models exist: the delta-delta Ct and the efficiency-corrected Ct model. Both models have their advantages and disadvantages in terms of simplification on the one hand and efficiency correction on the other.

Effects of varied EGCG and (+)-catechin concentrations on proinflammatory cytokines mrna expression in cona-stimulated primary white blood cell cultures.

EGCG [(-)-epigallocatechin gallate] and (+)-catechin hydrate are flavanoids, which are known as anticancer and healthy drugs. To test the immune modulatory effects of EGCG and catechin, various concentrations were tested on primary white blood cells (WBC) in cell cultures stimulated with the T-cell mitogen concanavalin A (ConA). WBC from dairy cows (1 x 10(6) cells/mL) were cultivated using RPMI medium with FCS and gentamycin.