Effects of supplementing direct-fed microbials on health and growth of preweaning Gyr × Holstein dairy calves.

This study aimed to evaluate the effects of direct-fed microbials (DFM) on health and growth responses of preweaning Bos indicus × Bos taurus (Gyr × Holstein) crossbred calves. Ninety newborn heifer calves (initial BW of 35 ± 4.0 kg) were used.

Development of an electrochemical DNA-based biosensor for the detection of the cardiovascular pharmacogenetic-altering SNP CYP2C9*3.

Cardiovascular diseases are among the major causes of mortality and morbidity. Warfarin is often prescribed for these disorders, an anticoagulant with inter and intra-dosage variability dose required to achieve the target international normalized ratio. Warfarin presents a narrow therapeutic index, and due to its variability, it can often be associated with the risk of hemorrhage, or in other patients, thromboembolism.

Development of a Novel Phagomagnetic-Assisted Isothermal DNA Amplification System for Endpoint Electrochemical Detection of .

The hitherto implemented detection techniques are cumbersome or require expensive non-portable instrumentation, hindering their transposition into on-time surveillance systems. The current work proposes a novel integrated system resorting to loop-mediated isothermal amplification (LAMP), assisted by a bacteriophage P100-magnetic platform, coupled to an endpoint electrochemical technique, towards expeditious detection. Molybdophosphate-based optimization of the bacterial phagomagnetic separation protocol allowed the determination of the optimal parameters for its execution (pH 7, 25 °C, 32 µg of magnetic particles; 60.

Opioids and constipation therapy in the last week of life: Their impact on patients, caregivers, and the location of death.

The use of opioids to control pain at the end of life may cause constipation, a symptom that can negatively influence the well-being of patients and caregivers. The main aim of this study was to evaluate the impact of constipation on symptomatic control and patients' overall quality of life at this stage. A particular focus was placed on opioids.

Perception of men perpetrators of violence about paternity.

Objectives: to learn about the perception of men in the judicial process due to spousal violence about paternity.

Methods: a descriptive, qualitative study with nine men in the judicial process due to spousal violence. Data were collected through interviews; the men validated the data, which were systematized by the categorical thematic content analysis, and analyzed in light of scientific texts that discuss the subject.

Phenolic Extraction of Leaves in DES: Characterization of the Extracts and Their Application in Methylcellulose Films for Food Packaging.

In this work, a qualitative study of the phenolic content of leaves (MO), extracted with deep eutectic solvents (DES) based on choline chloride (ChCl) with lactic acid (LA) or glycerol (GLY), was performed by high-resolution mass spectrometry (HPLC-DAD-ESI-MS). The two solvents (DES-LA and DES-GLY) extract similar classes of phenolics, and ten compounds were identified. The antioxidant profile was also studied (TPC, TFC, DPPH, FRAP, ORAC, and ABTS).

[Constituent elements of masculinity taught/learned in childhood and adolescence of men who are being criminally prosecuted for violence against women/partners].

The scope of this article was to understand the constituent elements of masculinity taught/learnt in childhood/adolescence and reproduced by men in criminal proceedings for conjugal violence. Oral Life History was used as a methodological reference. Interviews were conducted with 13 men in criminal proceedings for domestic violence.

Association between attention, nonverbal intelligence and school performance of school-age children with Autism Spectrum Disorder from a public health context in Brazil.

Background: Autism Spectrum Disorder (ASD) is characterized by impairments in social interaction, restricted and repetitive behaviour, interests or activities. Difficulties in a broad spectrum of cognitive skills is often present, including attentional processes and nonverbal intelligence, which might be related to academic difficulties.

Aims: In this study, the association between attentional skills and nonverbal intelligence to school performance of children with ASD was assessed.

Effects of the Reactive Moiety of Phenolipids on Their Antioxidant Efficiency in Model Emulsified Systems.

Our previous research was focused on the effects of hydrophobicity on the antioxidant (AO) efficiency of series of homologous antioxidants with the same reactive moieties. In this work we evaluate the antioxidant efficiency of hydrophobic phenolipids in 4:6 olive oil-in-water emulsions, with different phenolic moieties (derived from caffeic, 4-hydroxycinnamic, dihydrocaffeic acids, tyrosol and hydroxytyrosol), with alkyl chains of 8 and 16 carbons, and compare the antioxidant efficiency with that of the parent compounds. All catecholic phenolipids, in particular the C8 derivatives, have proven to be better antioxidants for the oxidative protection of emulsions than their parental compounds with octyl dihydrocafffeate being the most efficient (16-fold increase in relation to the control).

Meanings of intrafamily violence experienced in childhood/adolescence present in the discourses of men.

Objective: to unveil the meanings of intrafamily violence experienced in childhood and/or adolescence by men under legal proceedings due to conjugal violence.

Method: a qualitative research, based on Thematic Oral History and supported by Symbolic Interactionism. Participants were men under legal proceedings due to domestic violence who had experienced or witnessed intrafamily violence in childhood and/or adolescence.

Contributions of a social technology from the perspective of men being sued for gender violence.

Objectives: to discover what were the contributions of the Reflexive Group from the perspective of men being sued by marital violence, who participated in a social technology.

Methods: qualitative study, resulting from the evaluation stage of an action-research, based on the perspective of Critical Freedom and carried out with 44 men who were being sued in the Peace at Home Courts, in Salvador, Bahia, Brazil.

Results: the discourses of men being sued for marital violence showed that the reflections enabled them to share their experiences; understand their attitudes as disrespectful, violent, and liable in criminal courts; elaborate of strategies for a harmonious marital life; and become multipliers as a result of their willingness to socialize the information and knowledge that emerged from their group.

Marital violence precipitating/intensifying elements during the Covid-19 pandemic.

The study aims to identify marital violence precipitating/intensifying elements during the COVID-19 pandemic. This is a narrative review of the literature, and the search was carried out in May 2020. We employed the PubCovid-19 platform, which is indexed in the United States National Library of Medicine (PubMed) and the Excerpta Medica (EMBASE) database.

Social attributes of the male that incite the violence by intimate partner.

Objective: To analyze the social attributes of masculinity that incite the practice of conjugal violence.

Method: Oral Life History was used as a methodological reference. Interviews were conducted with 13 men facing criminal proceedings for conjugal violence linked to the 2nd Vara De Justiça pela Paz Em Casa (2nd Court of Justice for Peace at Home), in the municipality of Salvador, Bahia, Brazil, in April and July 2018.

Electrochemical immunosensor towards invasion-associated protein p60: An alternative strategy for Listeria monocytogenes screening in food.

This work reports the development of an electrochemical immunosensor for rapid, specific and decentralized detection of the invasion-associated protein p60 secreted by Listeria monocytogenes, a life-threatening foodborne pathogen. A disposable screen-printed electrode was used as transducer surface and monoclonal and polyclonal antibodies that specifically recognize Listeria monocytogenes p60 protein and Listeria spp. p60 proteins, respectively, were used as the sandwich immuno-pair.

Repercussions of family violence: oral history of adolescents.

Objective: To know the repercussions of the experience of family violence from the oral history of adolescents.

Method: Qualitative study based on the Oral History method, conducted from interviews with adolescents enrolled in a public school in Salvador, State of Bahia, Brazil. The data were systematized according to the thematic analysis and supported by theoretical references on family violence and adolescence.

Development of a disposable paper-based potentiometric immunosensor for real-time detection of a foodborne pathogen.

This work reports a new paper-based sensing platform and its application in a label-free potentiometric immunosensor for Salmonella typhimurium detection based on the blocking surface principle. A paper-based strip electrode was integrated with a filter paper pad which acted as a reservoir of the internal solution. The design offers a convenient platform for antibody immobilization and sampling, proving also that is a simple and affordable methodology to control an ionic flux through a polymer membrane.

In situ formation of gold nanoparticles in polymer inclusion membrane: Application as platform in a label-free potentiometric immunosensor for Salmonella typhimurium detection.

Polymeric ion selective electrodes are highly sensitive to changes in zero current ion flow and this offers a route to signal amplification in label-free potentiometric immunosensors. In this work, a label-free potentiometric immunosensor toward Salmonella typhimurium (ST) assembled in a home-made pipette-tip electrode is described. The signal-output amplification was implemented on a gold nanoparticle polymer inclusion membrane (AuNPs-PIM) which was used as sensing platform and for antibody immobilization.

Electrochemical biosensors for Salmonella: State of the art and challenges in food safety assessment.

According to the recent statistics, Salmonella is still an important public health issue in the whole world. Legislated reference methods, based on counting plate methods, are sensitive enough but are inadequate as an effective emergency response tool, and are far from a rapid device, simple to use out of lab. An overview of the commercially available rapid methods for Salmonella detection is provided along with a critical discussion of their limitations, benefits and potential use in a real context.

[The (un)receptive experiences of female rape victims who seek healthcare services].

Objective: To know the structure and functioning of healthcare services from the perspective of women who have suffered rape.

Method: A qualitative study conducted with 11 women who experienced rape, monitored in a maternity in the state of Alagoas, Brazil. Data were systematically based on content analysis.

Breast cancer metastasis to the pituitary gland.

Metastatic tumors to the pituitary gland are an unusual complication typically seen in elderly patients with diffuse malignant disease. Breast and lung are the commonest sites of the primary tumor. Prognosis of patients with breast cancer metastasis is poor and depends on the primary neoplastic extension.

MIP-graphene-modified glassy carbon electrode for the determination of trimethoprim.

A novel sensitive electrochemical sensor was developed by electropolymerization of pyrrole (PY) and molecularly imprinted polymer (MIP) which was synthesized onto a glassy carbon electrode (GCE) in aqueous solution using cyclic voltammetry in the presence of Trimethoprim (TMP) as template molecules. Furthermore, a previous electrode modification was performed by deposition of a suspension of graphene on the electrode's surface. The performance of the imprinted and non-imprinted (NIP) films was evaluated by impedance spectroscopy (EIS) and cyclic voltammetry (CV) of a ferric solution.

Simultaneous electrochemical determination of arsenic, copper, lead and mercury in unpolluted fresh waters using a vibrating gold microwire electrode.

In this work, a simple, rapid, reliable and low cost method for simultaneous electrochemical determination of As, Cu, Hg and Pb ions, on a vibrating gold microwire electrode combined with stripping voltammetry, is described for the first time. The multi-element detection was performed in the presence of oxygen by differential pulse anodic stripping voltammetry (DPASV) in HCl 0.1 M with NaCl 0.

Array of potentiometric sensors for simultaneous analysis of urea and potassium.

Urea biosensors based on urease immobilized by crosslinking with BSA and glutaraldehyde coupled to ammonium ion-selective electrodes were included in arrays together with potassium, sodium and ammonium PVC membrane ion-selective electrodes. Multivariate calibration models based on PCR and PLS2 were built and tested for the simultaneous determination of urea and potassium. The results show that it is possible to obtain PCR and PLS2 calibration models for simultaneous determination of these two species, based on a very small set of calibration samples (nine samples).