Use of RNA Immunoprecipitation Method for Determining RNAHfq Protein Associations In Vivo.

Background: Soil bacterium () forms an endosymbiotic partnership with () roots which results in root nodules. The bacteria live within root nodules where they function to fix atmospheric N and supply the host plant with reduced nitrogen. The bacterial RNA-binding protein Hfq (Hfq) is an important regulator for the effectiveness of the nitrogen fixation.